Abstract
Biopanning, a common affinity selection approach in phage display, has evolved numerous ligands for diagnosis, imaging, delivery, and therapy applications. However, traditional biopanning has suffered from time-consuming processes, highly-repetitive procedures and labor-intensive manual operation. Herein, a highly integrated and automated biopanning platform (Auto-Panning) is proposed. Based on digital microfluidics (DMF), biopanning processes are integrated on a chip with highly reproducible, precise, automated liquid manipulation. Therefore, 3 rounds of Auto-Panning can be accomplished within 16 h, instead of nearly a week of complicated manual operations. Auto-Panning has been used to evolve a specific peptide against cancer biomarker EphA2 with excellent cellular penetrating ability and significant invasion suppression biofunction, successfully demonstrating the practicality of the platform. Overall, as an automated programmable molecular screening platform, Auto-Panning will further promote the discovery and applications of novel ligands.
References
Feb 4, 1994·Science·E J Rebar, C O Pabo
Feb 19, 1999·BioTechniques·S J McConnellD G Spinella
Nov 2, 2001·Nature Medicine·R J GiordanoW Arap
Feb 1, 2002·Nature Medicine·Wadih ArapRenata Pasqualini
Dec 13, 2003·Neoplasia : an International Journal for Oncology Research·Kimberly A Kelly, David A Jones
Jun 9, 2004·Applied and Environmental Microbiology·G O'FlynnA Coffey
Jul 14, 2004·Biochemical and Biophysical Research Communications·Mark S DuxburyEdward E Whang
Aug 16, 2005·Cancer Cell·Madhu MacraeFrank McCormick
Mar 9, 2006·Journal of Cell Science·Monique DailElena B Pasquale
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Apr 8, 2009·Lab on a Chip·Yanli LiuH Tom Soh
Feb 16, 2010·Analytical Chemistry·Zhishan HuaMichael G Pollack
Feb 23, 2010·Chemical Reviews·Susan L Deutscher
Sep 15, 2010·Biomedical Microdevices·Sam H AuAaron R Wheeler
Feb 15, 2012·Journal of Medicinal Chemistry·Si WangMaurizio Pellecchia
Apr 25, 2012·Annual Review of Analytical Chemistry·Kihwan ChoiAaron R Wheeler
Sep 28, 2012·Analytical Chemistry·Alphonsus H C NgAaron R Wheeler
Jan 23, 2014·Nature Communications·Jin-Woo OhSeung-Wuk Lee
Apr 12, 2016·Biotechnology Journal·Yuyu TanChaoyong J Yang
Feb 18, 2017·Trends in Pharmacological Sciences·Giulia GuidottiDaniela Rossi
May 17, 2017·Chemical Science·Chao ChenBen Zhong Tang
Oct 20, 2017·Analytical and Bioanalytical Chemistry·Yuyu TanChaoyong Yang
Nov 9, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Daniela Kalafatovic, Ernest Giralt
Mar 24, 2018·Analytical Chemistry·Yang WangChaoyong Yang
Jun 8, 2018·Science Translational Medicine·Xianjun GaoHaiFang Yin
Jul 11, 2018·Lab on a Chip·Hugo SinhaSteve C C Shih
Aug 16, 2018·ACS Chemical Biology·Luca GambiniMaurizio Pellecchia
Nov 30, 2018·Biosensors & Bioelectronics·Fenxiang ZouChaoyong James Yang
Jan 12, 2019·Biosensors & Bioelectronics·Loïc CoudronMark C Tracey
Sep 19, 2019·Angewandte Chemie·George P Smith
Mar 24, 2020·Journal of Medicinal Chemistry·Gemma E MuddGavin S Bennett
May 20, 2020·Analytical Chemistry·Xing XuChaoyong Yang