PMID: 11914132Mar 27, 2002Paper

Auto-protective redox buffering systems in stimulated macrophages

BMC Immunology
Pierre-Jacques FerretDidier Fradelizi

Abstract

Macrophages, upon encounter with micro-organisms or stimulated by cytokines, produce various effector molecules aimed at destroying the foreign agents and protecting the organism. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are front line molecules exerting strong cytotoxic activities against micro-organisms and many cells, including macrophages themselves. Using cells of the murine macrophage cell line (RAW 264.7) stimulated in vitro with lipopolysaccharide (LPS) and/or interferon (IFN-gamma), which induce strong endogenous NO production, we examined by which mechanisms a fraction of activated macrophages protect themselves from nitrosative stress and manage to escape destruction? We observed that survivors (10-50% depending on the experiments) had acquired a resistant phenotype being capable to survive when further exposed in vitro to an apoptosis inducing dose of the NO donor compound DETA-NO. These cells expressed an increased steady-state levels of Mn SOD, CuZn SOD and catalase mRNA (130-200%), together with an increased activity of the corresponding enzymes. Intracellular concentration of glutathione was also increased (x 3.5 fold at 6 hours, still maintained x 5.2 fold at 48 hours). Neither mRNA for...Continue Reading

Citations

Oct 1, 2004·The Journal of Biological Chemistry·Sanjeev KumarCarolina Barillas-Mury
Jan 21, 2014·Journal of Biomedical Materials Research. Part a·Qiang-Song WangXi-Zheng Zhang
Mar 5, 2019·Journal of Applied Toxicology : JAT·Diego García-MendozaNico W van den Brink
Mar 15, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·De-yuan LiPu-yan Chen
Jan 24, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sei-Jung Lee, Kye-Taek Lim
Apr 22, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Constanze PietschWerner Kloas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.