Autoagglutinin produced by organ fragment cultures of mouse spleen

European Journal of Immunology
K F Jablonska


Mouse spleen fragment microcultures produced a heat-labile substance which agglutinated syngeneic erythrocytes. The substance reacted with an antigen present on some strains of mouse erythrocytes and on rat erythrocytes, but absent from other strains of mouse erythrocytes and from guinea pig, rabbit or sheep erythrocytes. Agglutination was inhibited by antisera to mouse immunoglobulins except for antisera specific to mouse IgM which enhanced agglutination. The agglutinin sedimented with 19 S Ig on ultracentrifugation and was destroyed by pretreatment with mercaptoethanol. Using a rosette assay increased numbers of cells, specifically binding syngeneic erythrocytes by means of a surface Ig receptor were demonstrated in spleen fragment microcultures. It is considered that the agglutinin is a noncomplement-activating IgM autoantibody and that the precursors of the cells producing the autoantibody are detected by the rosette assay.


Nov 1, 1975·Cellular Immunology·M Small, N Trainin
May 1, 1975·European Journal of Immunology·J C GluckmanP Cornillot
Aug 1, 1972·The Journal of Experimental Medicine·N R Klinman
Jan 1, 1974·Contemporary Topics in Immunobiology·A C Allison
Jun 1, 1969·Arthritis and Rheumatism·D M Weir, C J Elson
Sep 12, 1967·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D D McGregorJ L Gowans
Jun 13, 1973·Nature: New Biology·J H Playfair, S Marshall-Clarke
Oct 6, 1971·Nature: New Biology·I McConnell
Oct 22, 1965·Science·T Borsos, H J Rapp
Nov 1, 1966·The Journal of Experimental Medicine·A Globerson, R Auerbach
Mar 29, 1957·Science·H F DEUTSCH, J I MORTON

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