Autoantibodies against nucleoporin p62 constitute a novel marker of primary biliary cirrhosis

Gastroenterology
J Wesierska-GadekE Penner

Abstract

Patients with primary biliary cirrhosis (PBC) frequently produce autoantibodies against constituents of the nuclear envelope. We have recently observed a reactivity of PBC sera with a nuclear envelope antigen at approximately 60 kilodaltons. The aim of this study was to characterize the reactive antigen. Sera from 103 patients with liver disease were tested by immunoblotting after the separation of proteins by one- and two-dimensional polyacrylamide gel electrophoresis (PAGE) using proteins of whole nuclei and nuclear pore complexes (NPCs). A nuclear immunofluorescence staining with peripheral accentuation was observed in 25 of 43 sera from patients with PBC. In immunoblotting, the sera displaying a peripheral pattern reacted preferentially with two proteins. Twelve sera showed specificity to an antigen of 210 kilodaltons corresponding to gp210, and 14 sera recognized a protein band at 60 kilodaltons. The intensity of the reactive band at 60 kilodaltons was clearly stronger on blots with immobilized proteins of the NPC than on blots with nuclear proteins, thereby indicating that the sera targeted an antigen as being a component of the NPC. Immunoblotting performed after protein separation by two-dimensional PAGE showed that the...Continue Reading

Citations

Jun 1, 2012·Molecular & Cellular Proteomics : MCP·Chao-Jun HuYong-Zhe Li
Jan 31, 2008·Postgraduate Medical Journal·D E J Jones
Dec 29, 2000·Journal of Clinical Pathology·D E Jones
Sep 8, 2006·BMC Immunology·Vagia TsiakalouPanayiotis A Theodoropoulos
Jun 6, 2008·World Journal of Gastroenterology : WJG·Ana LleoM-Eric Gershwin
Aug 3, 2010·World Journal of Gastroenterology : WJG·Chao-Jun HuXuan Zhang
Dec 15, 2012·Expert Review of Gastroenterology & Hepatology·Maria I S AchenzaCarlo Selmi
Dec 3, 2011·Expert Review of Molecular Diagnostics·Alessandro GranitoLuigi Muratori
Nov 11, 2014·Expert Review of Clinical Immunology·Ahmad H AliKeith D Lindor
Jun 1, 2008·Expert Opinion on Medical Diagnostics·Eirini I Rigopoulou, George N Dalekos
Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J Neuberger, R Thomson
Nov 15, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D ZüchnerH Will
Apr 11, 2012·Autoimmunity Reviews·Carolina Duarte-ReyM Eric Gershwin
Dec 28, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Dimitrios P Bogdanos, Lars Komorowski
Oct 5, 2002·Clinics in Liver Disease·Francesco B BianchiLuigi Muratori
Apr 22, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Józefa Wesierska-GadekPietro Invernizzi
Oct 13, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Howard J Worman
Aug 15, 2006·Journal of Autoimmunity·Christopher A AokiM Eric Gershwin
Dec 15, 2010·Hepatitis Research and Treatment·Motoko Sasaki, Yasuni Nakanuma
May 12, 2000·Journal of Structural Biology·Y GruenbaumM Cohen
Sep 8, 2000·Baillière's Best Practice & Research. Clinical Gastroenterology·C P Strassburg, M P Manns
Feb 13, 2001·European Journal of Clinical Investigation·J MedinaR Moreno-Otero
Feb 20, 2003·The American Journal of Gastroenterology·Paolo MuratoriFrancesco B Bianchi
Nov 23, 2017·Hepatology International·Ana LleoM Eric Gershwin
Aug 12, 2015·World Journal of Gastrointestinal Pharmacology and Therapeutics·Elias Kouroumalis, George Notas
Apr 22, 2003·Journal of Cellular Biochemistry·Józefa Wesierska-GadekGerald Schmid
Aug 5, 2003·Journal of Cellular Biochemistry·Józefa Wesierska-GadekGerald Schmid
Nov 24, 2004·The American Journal of Gastroenterology·Manuel Romero-GómezUNKNOWN Spanish Group for the Study of Autoimmune Cholangitis
Jan 10, 2019·Annals of Laboratory Medicine·Alicja Bauer, Andrzej Habior
Oct 26, 2007·Journal of Cellular Biochemistry·Józefa Wesierska-GadekEdward Penner
Aug 2, 2014·Seminars in Immunopathology·Evaggelia LiaskouM Eric Gershwin
Jul 15, 2015·World Journal of Gastroenterology : WJG·Vasiliy Ivanovich Reshetnyak
Nov 2, 2013·World Journal of Hepatology·Ourania SfakianakiElias Kouroumalis
Mar 11, 2003·Clinical and Diagnostic Laboratory Immunology·Doris M KraemerHans-Peter Tony
Dec 1, 2012·Auto- Immunity Highlights·Andreas L KoutsoumpasEirini I Rigopoulou
Aug 1, 2013·Auto- Immunity Highlights·Takashi Himoto, Mikio Nishioka
Jul 12, 2017·Clinical & Translational Immunology·Ban-Hock Toh
Nov 6, 2003·Clinics in Liver Disease·Hiroto KitaM Eric Gershwin

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