Autoantibodies against the Second Extracellular Loop of M3R Do neither Induce nor Indicate Primary Sjögren's Syndrome

PloS One
Yan ChenXinhua Yu

Abstract

Anti-muscarinic acetylcholine type-3 receptor (anti-M3R) autoantibodies have been suggested to be pathogenic for primary Sjögren's syndrome (pSS), and the second extracellular loop of M3R is suspected to carry a disease-promoting epitope. In this study, we aimed to evaluate the pathogenicity of autoantibodies against peptides derived from the second extracellular loop of M3R in mice and to determine whether those autoantibodies could be used as biomarker for pSS. BALB/c mice were immunized with modified linear or cyclic peptides of the second extracellular loop of M3R. The function of exocrine glands was evaluated by measuring the secretion of saliva and tears. The histological evaluations were performed by using H&E staining or direct immunofluorescence staining. Autoantibodies against linear or cyclic peptides of the second extracellular loop of M3R in human and mice were determined using ELISA. Immunization induced mice to produce autoantibodies against the linear or cyclic peptides of the second extracellular loop of M3R, and those autoantibodies could bind onto salivary glands. However, those mice showed neither impairment in the secretion of tears or saliva nor histological abnormality in the exocrine glands. Furthermore,...Continue Reading

References

Jan 1, 1990·Annual Review of Pharmacology and Toxicology·E C HulmeN J Buckley
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·C P RobinsonM G Humphreys-Beher
Apr 26, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J M RobertsonB D Evavold
Oct 19, 2000·Arthritis and Rheumatism·K H NguyenM G Humphreys-Beher
May 15, 2002·Annals of the Rheumatic Diseases·C VitaliUNKNOWN European Study Group on Classification Criteria for Sjögren's Syndrome
Nov 12, 2009·Autoimmunity Reviews·Clio P Mavragani, Haralampos M Moutsopoulos
Feb 23, 2011·Journal of Visualized Experiments : JoVE·Sarah PringleRob P Coppes
Dec 18, 2013·Journal of Autoimmunity·Nikolaos C KyriakidisAthanasios G Tzioufas
Feb 4, 2014·Journal of Autoimmunity·Haralampos M Moutsopoulos
Mar 18, 2015·Current Pharmaceutical Design·Xinhua YuFrank Petersen

❮ Previous
Next ❯

Citations

Nov 10, 2017·Frontiers in Immunology·Junfeng ZhengXinhua Yu
Sep 29, 2018·International Journal of Molecular Sciences·Marta TrzeciakUmesh S Deshmukh
Feb 17, 2019·Rheumatology·Julie MielleJacques-Olivier Pers
Jun 15, 2021·Frontiers in Immunology·Marvin J FritzlerMichael Mahler

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
Flow cytometry
FACS
ELISAs

Software Mentioned

SPSS

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.