Autoantibodies detectable in the sera of silicosis patients. The relationship between the anti-topoisomerase I antibody response and HLA-DQB1*0402 allele in Japanese silicosis patients

The Science of the Total Environment
A UekiF Hydoh

Abstract

Eighty-one Japanese silicosis patients and 66 healthy volunteers were analyzed for autoantibodies by ELISA, and HLA-genotyping using the PCR-RFLP method was performed. Anti-topoisomerase I (anti-topo I) autoantibodies were detected in seven patients without any clinical features of autoimmune diseases such as progressive systemic sclerosis (PSS), although anti-topo I have been mostly reported in PSS patients. Antibodies directed to RNP, ssDNA, dsDNA and cent-B were not detected among the anti-topo I positive patients. The indirect immunofluorescent staining pattern of Hep-2 cells with the sera of anti-topo I positive silicosis patients demonstrated the typical mode of anti-topo I autoantibodies observed in the patients with PSS. The allelic frequency of HLA-DQB1*0402 was significantly higher in anti-topo I positive patients (28.6%) than in anti-topo I negative patients (1.5%, P < 0.001) or healthy controls (0.8%, P<0.001). HLA-DQB1*0301, DQB1*0601 and DPB1*1801 alleles were more frequently detected in anti-topo I positive patients than in the patients without anti-topo I or in healthy volunteers, but no significant difference was observed. DQB1 allele is associated with the induction of anti-topo I autoantibodies in Japanese si...Continue Reading

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Citations

Aug 26, 2009·Rheumatology International·Gabriel ZaghiMarilia Barreto Silva
Oct 5, 2010·Nihon eiseigaku zasshi. Japanese journal of hygiene·Naoko KumagaiTakemi Otsuki
Jul 8, 2009·Arhiv za higijenu rada i toksikologiju·Omid AminianMaria Mazaheri
Aug 20, 2014·Environmental Health and Preventive Medicine·Suni LeeTakemi Otsuki
Feb 9, 2017·Current Opinion in Allergy and Clinical Immunology·Suni LeeTakemi Otsuki
Jan 20, 2011·International Journal of Immunopathology and Pharmacology·H HayashiT Otsuki
Jun 8, 2017·Experimental and Therapeutic Medicine·Suni LeeTakemi Otsuki

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