Autoantibodies specific to hnRNP K: a new diagnostic marker for immune pathophysiology in aplastic anemia

Annals of Hematology
Zhirong QiShinji Nakao

Abstract

To identify a new diagnostic marker for the immune pathophysiology of aplastic anemia (AA), we screened sera of immune-mediated AA patients for the presence of antibodies (Abs) specific to proteins derived from a leukemia cell line UT-7 using two-dimensional electrophoresis followed by immunoblotting. The target proteins were identified by peptide mass fingerprinting. Heterogeneous nuclear ribonucleoprotein (hnRNP) K was identified as a novel autoantigen. An enzyme-linked immunosorbent assay revealed high titers of anti-hnRNP K Abs in 85 (31%) of 273 patients with AA. Sixty-four patients received antithymocyte globulin and cyclosporine after undergoing screening for anti-hnRNP K Ab, anti-DRS-1 Ab, anti-moesin Ab, and paroxysmal nocturnal hemoglobinuria (PNH)-type cells. Twenty (87%) of 23 patients with the presence of anti-hnRNP K Abs responded to the immunosuppressive therapy (IST), while 19 (46%) of 41 patients without the presence of anti-hnRNP K Abs responded. A multivariate analysis showed only PNH-type cells and anti-hnRNP K Abs to be significant factors for the prediction of a good response to IST. The detection of anti-hnRNP K Abs as well as PNH-type cells may therefore be useful for diagnosing the immune pathophysiolog...Continue Reading

References

Apr 11, 1988·FEBS Letters·A DangliC E Sekeris
Feb 17, 1997·FEBS Letters·K BomsztykJ Ostrowski
Oct 1, 1996·Molecular Immunology·M WagatsumaH Watanabe
Feb 5, 1998·Journal of Mass Spectrometry : JMS·J R Yates
Mar 8, 2000·Acta Haematologica·B M Camitta
Dec 31, 2000·The Journal of Biological Chemistry·M MandalR Kumar
Feb 5, 2002·Electrophoresis·T A NymanR Lahesmaa
Jul 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ruth FritschGünter Steiner
Jul 11, 2003·The New England Journal of Medicine·Thomas BergerMarkus Reindl
Jun 2, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Karol BomsztykJerzy Ostrowski
Jun 12, 2004·The Journal of Investigative Dermatology·David A JonesThomas S Kupper
Apr 5, 2005·Autoimmunity·Roberto CaporaliCarlomaurizio Montecucco
Nov 30, 2005·American Journal of Hematology·Per Boye Hansen, Anne M Falensteen Lauritzen
Jan 13, 2006·British Journal of Cancer·K Klimek-TomczakJ Ostrowski
Jan 3, 2007·Experimental Hematology·Chiharu SugimoriShinji Nakao

❮ Previous
Next ❯

Citations

Feb 7, 2012·Archivum Immunologiae Et Therapiae Experimentalis·David Gómez-AlmaguerGuillermo J Ruiz-Arguelles
Aug 15, 2012·Acta Haematologica·Jan LakotaMartin Stern
Nov 3, 2012·British Journal of Haematology·Maki GotoNaoki Watanabe
Feb 17, 2015·Clinical and Experimental Immunology·Y Zeng, E Katsanis
Dec 9, 2015·Journal of Clinical Pathology·Jonathan R Kerr
Jun 1, 2020·Scandinavian Journal of Immunology·Na XiaoZonghong Shao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.