PMID: 11935433Apr 6, 2002Paper

Autoantibodies specificity in acute anterior uveitis according to the presence of the HLA-B27 allele

Ocular Immunology and Inflammation
V F TrevisaniL E Andrade

Abstract

To study HLA-B27(+) and HLA-B27(-) patients with AAU with respect to the frequency and specificity of organ-specific and non-organ-specific autoantibodies. Fifty-seven consecutive patients with AAU were subjected to ophthalmologic and rheumatologic evaluation as well as to autoantibody determination: antinuclear antibodies (ANA), rheumatoid factor (RF), anticardiolipin, anti-smooth muscle, and anti-parietal cell antibodies, and immunoblot for antibodies to HeLa cells and to bovine iris extract. HLA-B27 was determined by a microlymphocytotoxicity assay. Statistical analysis employed chi-square test, Fisher's exact test, and McNemar test. Thirty-four patients had the HLA-B27 allele (B27(+)/AAU) and 23 did not (B27(-)/AAU). ANA, RF, anticardiolipin, and anti-parietal cell antibodies appeared in low frequency. No patient presented anti-smooth muscle antibody. Immunoblot showed a high frequency of antibodies to HeLa cell proteins in B27(-)/AAU patients with predominant reactivity at 46 kDa and 56 kDa. In contrast, sera from B27(+)/AAU patients reacted poorly against HeLa cell antigens. Immunoblot with bovine iris extract showed a significant frequency of antibodies in both groups, with a predominant response to antigens with an esti...Continue Reading

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Related Concepts

Acute Disease
Alleles
Antibody Specificity
Fluorescent Antinuclear Antibodies
Autoantibodies
Autoantigens
Diphosphatidylglycerols
SDS-PAGE
Enzyme-Linked Immunosorbent Assay
HeLa Cells

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