PMID: 9434799Jan 22, 1998Paper

Autoantibodies to a novel early endosome antigen 1

Clinical Immunology and Immunopathology
R L WaiteB H Toh

Abstract

Autoantibodies to EEA1, an antigen on early endosomes, were first reported in the serum of a patient with subacute cutaneous lupus erythematosus (SCLE). Here we have examined 38 sera selected for investigation of autoantibodies to EEA1 on the basis of cytoplasmic vesicle-like reactivity by immunofluorescence. Ten of the sera were reactive to a HeLa cell protein of approximately the same M(r) as human EEA1. Eight of these sera belonged to the IgG1 subclass. Five of the sera reacted with fusion proteins incorporating either the amino (from amino acids 1 to 209) or the carboxyl (incorporating the most C-terminal 300 amino acids) terminus of the human EEA1 protein. Antigens reactive with these 5 sera colocalized with internalized transferrin receptors, indicating their association with early endosomes. The other 5 sera which did not react to both fusion proteins did not colocalize with internalized transferrin receptors. We conclude that 5 of the 38 patients (13%) have autoantibodies to EEA1. None of these patients have SCLE, but have generalized joint pain, polyarthritis, rheumatoid arthritis, or circulating rheumatoid factors.

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Citations

Feb 14, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Laura M StintonMarvin J Fritzler
May 18, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Laura M StintonMarvin J Fritzler
Nov 5, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Sanja SelakMarvin J Fritzler
Sep 22, 1999·European Journal of Biochemistry·J CallaghanH Stenmark
Mar 6, 2002·The Journal of Cell Biology·David SheffIra Mellman
Jun 4, 2005·Clinical and Experimental Immunology·K DunsterJ W Sentry
Feb 21, 2006·Clinical and Experimental Immunology·C C F C LaurinoL E C Andrade
Mar 1, 2003·Modern Rheumatology·T KakinumaJ W Sentry
Aug 14, 2003·Biochimica Et Biophysica Acta·Alison K Gillingham, Sean Munro
Sep 24, 2021·PLoS Computational Biology·Andreas Haahr LarsenMark S P Sansom

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