Abstract
Autoantibodies to EEA1, an antigen on early endosomes, were first reported in the serum of a patient with subacute cutaneous lupus erythematosus (SCLE). Here we have examined 38 sera selected for investigation of autoantibodies to EEA1 on the basis of cytoplasmic vesicle-like reactivity by immunofluorescence. Ten of the sera were reactive to a HeLa cell protein of approximately the same M(r) as human EEA1. Eight of these sera belonged to the IgG1 subclass. Five of the sera reacted with fusion proteins incorporating either the amino (from amino acids 1 to 209) or the carboxyl (incorporating the most C-terminal 300 amino acids) terminus of the human EEA1 protein. Antigens reactive with these 5 sera colocalized with internalized transferrin receptors, indicating their association with early endosomes. The other 5 sera which did not react to both fusion proteins did not colocalize with internalized transferrin receptors. We conclude that 5 of the 38 patients (13%) have autoantibodies to EEA1. None of these patients have SCLE, but have generalized joint pain, polyarthritis, rheumatoid arthritis, or circulating rheumatoid factors.
References
Aug 1, 1992·Lupus·E FonsecaD Pascual
Sep 1, 1992·The Journal of Clinical Investigation·G SteinerJ S Smolen
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B H TohD C Humphris
Dec 1, 1987·Alcoholism, Clinical and Experimental Research·R C BakerR A Deitrich
Mar 1, 1986·Arthritis and Rheumatism·S CohenR D Sontheimer
Apr 1, 1981·Journal of the American Academy of Dermatology·J N Gilliam, R D Sontheimer
Aug 1, 1995·Current Opinion in Cell Biology·J Gruenberg, F R Maxfield
Apr 1, 1994·Immunology and Cell Biology·J KooyP A Gleeson
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·T MimoriM Akizuki
Sep 1, 1993·The Journal of Clinical Investigation·M SimonG Serre
Apr 1, 1995·The Journal of Clinical Investigation·N DesprésH A Ménard
Jan 1, 1994·Autoimmunity·J P Yeo, B H Toh
Mar 5, 1998·Immunology·V M GitlitsB H Toh
Citations
Feb 14, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Laura M StintonMarvin J Fritzler
May 18, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Laura M StintonMarvin J Fritzler
Nov 5, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Sanja SelakMarvin J Fritzler
Sep 22, 1999·European Journal of Biochemistry·J CallaghanH Stenmark
Apr 16, 2002·Molecular Biology of the Cell·Theophany EystathioyMarvin J Fritzler
Dec 21, 2000·Clinical and Experimental Immunology·S SelakM J Fritzler
Mar 6, 2002·The Journal of Cell Biology·David SheffIra Mellman
Nov 1, 2007·Expert Opinion on Medical Diagnostics·Marvin J Fritzler
Jun 4, 2005·Clinical and Experimental Immunology·K DunsterJ W Sentry
Jul 27, 2007·Traffic·Canhong CaoMary-Pat Stein
Feb 21, 2006·Clinical and Experimental Immunology·C C F C LaurinoL E C Andrade
Nov 23, 2006·Neuroscience·S SelakJ Lerma
Jul 5, 2001·Journal of Autoimmunity·Y YoshidaU Yamashita
Mar 1, 2003·Modern Rheumatology·T KakinumaJ W Sentry
Feb 26, 2016·Experimental Dermatology·Ryuhei NishikawaTakashi Hashimoto
Apr 10, 2019·Scientific Reports·Alberto Baroja-MazoPablo Pelegrín
Sep 22, 2018·Clinical Rheumatology·Martin BromCecilia Nora Pisoni
Aug 14, 2003·Biochimica Et Biophysica Acta·Alison K Gillingham, Sean Munro
Jan 4, 2001·European Journal of Cell Biology·Y TsukadaB H Toh
May 10, 2002·European Journal of Cell Biology·Kate DunsterJohn W Sentry
Sep 24, 2021·PLoS Computational Biology·Andreas Haahr LarsenMark S P Sansom