PMID: 7543435Aug 1, 1995Paper

Autoantibodies to BCOADC-E2 in patients with primary biliary cirrhosis recognize a conformational epitope

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
P S LeungM E Gershwin

Abstract

Primary biliary cirrhosis (PBC) is an autoimmune disease of liver associated with a unique serologic response to mitochondrial autoantigens. Many of the autoantigens recognized by autoantibodies in PBC are members of the 2-oxo-acid dehydrogenase complex. The two major autoantigens are the E2 component of the pyruvate dehydrogenase complex (PDC-E2) and the E2 component of the branched chain 2-oxo-acid dehydrogenase complex (BCOADC-E2). The autoantibody response to PDC-E2 has been mapped to one immunodominant epitope, which consists of both linear and conformational components. The presence of a single immunodominant epitope in PDC-E2 is unusual when contrasted to the immune response to autoantigens in other human autoimmune diseases. We have mapped the epitope recognized by antimitochondrial autoantibodies (AMA) specific to BCOADC-E2 in patients with PBC by taking advantage of the full-length bovine BCOADC-E2 complementary DNA (cDNA) and a series of expression clones spanning the entire molecule. Reactivity to the various expression clones was studied by immunoblotting, enzyme-linked immunosorbent assay (ELISA), as well as selective absorption of patient sera by expressed protein fragments. Autoantibodies to BCOADC-E2 map within...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F H PettitL J Reed
Dec 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P S LeungM E Gershwin
Jan 1, 1990·Springer Seminars in Immunopathology·I R Mackay, M E Gershwin
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S P FusseyS J Yeaman
Sep 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J Lindenborn-FotinosP A Berg
Aug 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C D SurhM E Gershwin
Aug 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·O F JamesM F Bassendine
Sep 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G R FlanneryH Baum
Sep 1, 1989·Immunology Today·I R Mackay, M E Gershwin
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·I SuzukaT Moriuchi
May 25, 1989·The New England Journal of Medicine·J Van de WaterM E Gershwin
Dec 1, 1981·The Journal of Experimental Medicine·Y TakasakiE M Tan
Sep 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S ChaM E Gershwin
Dec 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J QuinnS J Yeaman

❮ Previous
Next ❯

Citations

Mar 21, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A MattaliaM E Gershwin
Jan 19, 2011·Clinical Reviews in Allergy & Immunology·Marco FolciCarlo Selmi
Sep 16, 2003·Journal of Hepatology·David E J Jones
Jun 2, 1998·Free Radical Biology & Medicine·J BustamanteB H Rihn
Nov 5, 2003·Clinics in Liver Disease·Dimitrios-Petrou BogdanosDiego Vergani
Sep 1, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P S Leung, K H Chu
Dec 9, 1998·Clinical and Experimental Immunology·N PelliD Vergani
Feb 13, 2001·European Journal of Clinical Investigation·J MedinaR Moreno-Otero
Oct 26, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A TanakaM E Gershwin
Oct 5, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M SasakiM E Gershwin
Jan 31, 2008·Postgraduate Medical Journal·D E J Jones
Dec 29, 2000·Journal of Clinical Pathology·D E Jones
Jul 16, 2009·Seminars in Immunopathology·Simon HohenesterUlrich Beuers
Mar 15, 2014·World Journal of Gastroenterology : WJG·Satoshi YamagiwaYutaka Aoyagi
Dec 2, 2015·Experimental and Therapeutic Medicine·Minoru TomizawaNaoki Ishige
Mar 16, 2007·Journal of Autoimmunity·Roman Rieger, M Eric Gershwin
Dec 22, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Eric Gershwin, Ian R Mackay
Apr 14, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Guanghua RongM Eric Gershwin
Jun 13, 2006·Cellular Immunology·Xiao-Song HeM Eric Gershwin
Apr 9, 2001·Clinics in Liver Disease·P Angulo, K D Lindor
Oct 13, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Robert P MyersHude Quan
Dec 15, 2010·Hepatitis Research and Treatment·Motoko Sasaki, Yasuni Nakanuma
Oct 14, 2011·International Journal of Hepatology·Motoko Sasaki, Yasuni Nakanuma
Oct 14, 2011·International Journal of Hepatology·Koichi TsuneyamaYasuni Nakanuma
Apr 29, 1999·Clinical Immunology : the Official Journal of the Clinical Immunology Society·S QuarantaM E Gershwin
Sep 8, 2000·Baillière's Best Practice & Research. Clinical Gastroenterology·A NishioM E Gershwin
Sep 8, 2000·Baillière's Best Practice & Research. Clinical Gastroenterology·C P Strassburg, M P Manns
Jul 25, 2006·Toxicology·Dominic P Williams
Sep 18, 2010·Chemico-biological Interactions·B K ParkD P Williams

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.