Autoantibodies to GAD65 and IA-2 in canine diabetes mellitus

Veterinary Immunology and Immunopathology
Lucy J DavisonBrian Catchpole

Abstract

Diabetes mellitus in dogs shares many characteristics with the human type 1 disease and virtually all diabetic dogs require insulin therapy to control hyperglycaemia. Insulin deficiency is suspected to result from immune-mediated destruction of pancreatic beta cells in some cases. Human patients suffering from Type 1A (immune-mediated) diabetes or latent autoimmune diabetes of the adult (LADA) demonstrate circulating autoantibodies against the 65kDa isoform of glutamic acid decarboxylase (GAD65) and/or insulinoma antigen-2 (IA-2). The aims of the current study were to develop radio-immunoassays to detect serum antibodies against recombinant canine GAD65 and IA-2 and to identify diabetic dogs showing serological evidence of autoreactivity to these pancreatic beta cell antigens. Canine GAD65 and the 3' end of IA-2 (coding for amino acids 771-979 of the intracellular domain) were amplified by PCR from cDNA prepared from canine insulinoma tissue and cloned into the pCRII vector. The canine sequences were later confirmed by identifying GAD2 and PTPRN genes from the dog genome assembly. Recombinant (35)S-methionine-radiolabelled canine GAD65 and IA-2 (771-979) proteins were used in radio-immunoprecipitation assays to screen sera from...Continue Reading

References

May 1, 1992·Veterinary Immunology and Immunopathology·M Hoenig, D L Dawe
Mar 1, 1986·Veterinary Immunology and Immunopathology·D M Haines
Apr 1, 1967·Diabetologia·W Gepts, D Toussaint
Nov 15, 2002·Molecular and Cellular Endocrinology·M Hoenig
Dec 18, 2003·Annals of the New York Academy of Sciences·Eiji KawasakiKatsumi Eguchi
Apr 15, 2005·The Veterinary Record·L J DavisonB Catchpole
Sep 10, 2005·Diabetologia·B CatchpoleL J Davison
Aug 19, 2007·The Journal of Small Animal Practice·P J WatsonM E Herrtage
Jan 17, 2008·Journal of Veterinary Internal Medicine·Alexander J GermanVincent Biourge

❮ Previous
Next ❯

Citations

Jul 2, 2014·The Journal of Endocrinology·Richard W Nelson, Claudia E Reusch
Sep 22, 2010·Research in Veterinary Science·Lucy J DavisonBrian Catchpole
May 18, 2010·The Veterinary Clinics of North America. Small Animal Practice·Laurel J Gershwin
Nov 9, 2010·Journal of Veterinary Internal Medicine·T FallO Kämpe
Mar 4, 2010·Tissue Antigens·A D ShortW E R Ollier
Jun 2, 2016·Endocrinología y nutrición : órgano de la Sociedad Española de Endocrinología y Nutrición·Yeray Brito-CasillasAna María Wägner
Jul 28, 2016·Journal of Veterinary Internal Medicine·C GilorS P DiBartola
Jun 10, 2015·PloS One·Emily J ShieldsJake A Kushner
Feb 9, 2017·Journal of Veterinary Internal Medicine·L J DavisonC A O'Callaghan
Jun 8, 2014·The Journal of Biological Chemistry·Yolanda F OteroOwen P McGuinness
Oct 8, 2014·Australian Veterinary Journal·T JohnstoneC T Mooney
Jan 15, 2015·The Journal of Small Animal Practice·L J Davison
Feb 6, 2020·The Journal of Small Animal Practice·M Lester, A L O'Kell
Nov 22, 2020·Naunyn-Schmiedeberg's Archives of Pharmacology·Xinghao JiangGuiping Ren
Feb 24, 2021·Veterinary Medicine and Science·Yeray Brito-CasillasAna M Wägner
Mar 2, 2021·The Veterinary Journal·Alice L DenyerUNKNOWN Canine Diabetes Genetics Partnership

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diabetes & Tolerance

Patients with type I diabetes lack insulin-producing beta cells due to the loss of immunological tolerance and autoimmune disease. Discover the latest research on targeting tolerance to prevent diabetes.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Adenoma, Islet Cell

Islet Cell Adenoma arises in the islet cells, which are insulin producing cells of the pancreas. These tumors can be either malignant or benign. Discover the latest research on Islet Cell Adenoma here.