Autoantibodies to insulin and to proinsulin in type 1 diabetic patients and in at-risk probands differentiate only little between both antigens

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme
H KeilackerK D Kohnert

Abstract

To answer the question whether insulin or proinsulin would be the true antigen for both insulin and proinsulin autoantibodies, displacement experiments of 125I-insulin and -proinsulin binding with both unlabeled antigens were performed in sera of four groups of antibody-positive probands: first-degree relatives of Type 1 diabetic patients, pre-Type 1 diabetic persons, recent-onset Type 1 diabetic patients, insulin-treated Type 1 diabetic patients. In subjects who were primarily screened to constitute these groups, prevalences of insulin and proinsulin autoantibodies were nearly identical. In antibody-positive sera, 125I-insulin and -proinsulin binding values in general were closely correlated to each other with regression coefficients near 1.0. In all groups of probands, mean values of 125I-insulin and -proinsulin binding did not significantly differ. With the exception of a few sera, insulin and proinsulin antibodies differentiated only little between both antigens. Epitopes of the insulin molecule are therefore preferred. Nevertheless, insulin and proinsulin autoantibodies are not completely identical nor are insulin autoantibodies merely a subgroup of proinsulin autoantibodies: In each group, in the mean, insulin antibodies ...Continue Reading

Citations

Sep 13, 2003·Journal of Immunological Methods·Silvina N ValdezEdgardo Poskus
Jul 10, 2003·Autoimmunity Reviews·Parth NarendranLeonard C Harrison
Jan 1, 1996·International Journal of Clinical & Laboratory Research·K D KohnertD Michaelis
Jun 16, 2010·Critical Reviews in Clinical Laboratory Sciences·Thomas R CsorbaMorley D Hollenberg
Jul 3, 2002·Endocrinology and Metabolism Clinics of North America·Alberto Pugliese
Mar 29, 2002·Diabetes/metabolism Research and Reviews·Alberto Pugliese, Donatella Miceli
May 17, 2005·Diabetes/metabolism Research and Reviews·Bernd FrankeTerry J Wilkin
Feb 20, 2003·Diabetes/metabolism Research and Reviews·Parth NarendranColin M Dayan
Oct 3, 1999·Annals of Clinical Biochemistry·P M Clark
Apr 22, 2021·Endocrine Reviews·Michelle SoCarla J Greenbaum

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diabetes & Tolerance

Patients with type I diabetes lack insulin-producing beta cells due to the loss of immunological tolerance and autoimmune disease. Discover the latest research on targeting tolerance to prevent diabetes.