Autoantibodies to the extracellular domain of the calcium sensing receptor in patients with acquired hypoparathyroidism

The Journal of Clinical Investigation
Y LiN Maclaren

Abstract

Acquired hypoparathyroidism (AH) has been considered to result from an autoimmune process but the self-antigens have not been identified. We studied 25 patients with AH, of which 17 had type I autoimmune polyglandular syndrome and 8 had AH associated with autoimmune hypothyroidism. Five of 25 (20%) AH sera reacted to a membrane-associated antigen of 120-140 kD in human parathyroid gland extracts using immunoblot analysis. This is the exact size of the calcium sensing receptor (Ca-SR). The AH sera were then tested by immunoblot using a membrane fraction of HEK-293 cells transfected with Ca-SR cDNA. Eight of 25 (32%) AH sera reacted to a 120-140-kD protein, which closely matched that recognized by the anti-Ca-SR IgG raised in rabbits. The Ca-SR cDNA was translated in vitro into two parts in order to identify the antigenic epitopes. By using this technique, 14 of 25 (56%) AH sera were positive to the extracellular domain of the Ca-SR, whereas none of the AH patients sera reacted to the intracellular domain. The reactivity of the positive sera was completely removed after pre-absorption with the Ca-SR containing membranes. Sera from 50 patients with various other autoimmune diseases as well as 22 normal controls were also tested, a...Continue Reading

References

Jun 1, 1988·The Journal of Clinical Endocrinology and Metabolism·P AhonenJ Perheentupa
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A FattorossiM L Brandi
Oct 1, 1986·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J T PosillicoE M Brown
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M L BrandiL A Fitzpatrick
Apr 30, 1984·Biochemical and Biophysical Research Communications·J M Gonzalez-RosM Martinez-Carrion
Nov 1, 1982·Journal of Neuropathology and Experimental Neurology·G FumagalliJ Lindstrom
Aug 1, 1981·Experimental Neurology·A DesoukiM E Eldefrawi
Oct 15, 1994·Lancet·Y H SongN Maclaren
Apr 15, 1993·Nature

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Citations

May 1, 1996·Immunology Today·Y H SongN K Maclaren
Jul 17, 2013·Best Practice & Research. Clinical Endocrinology & Metabolism·Fadil M Hannan, Rajesh V Thakker
Aug 30, 2005·Comptes rendus biologies·Tristan Bouschet, Jeremy M Henley
Jan 16, 1999·Baillière's Clinical Gastroenterology·P Obermayer-Straub, M P Manns
Aug 2, 2000·Trends in Endocrinology and Metabolism : TEM·P PetersonK J Krohn
Sep 3, 2004·The New England Journal of Medicine·Matthias Schott, Werner A Scherbaum
Jul 23, 2004·The New England Journal of Medicine·J Carl PallaisEdward M Brown
Mar 7, 2008·The New England Journal of Medicine·Mohammad AlimohammadiOlle Kämpe
Sep 8, 2005·Southern Medical Journal·Wassim McHaylehEric S Nylen
Aug 1, 1997·Acta Paediatrica Japonica; Overseas Edition·T Yasuda, H Niimi
Oct 26, 2007·Pediatric Dermatology·Nicole LeBoeufSophie Worobec
Jun 12, 2010·International Journal of Rheumatic Diseases·Maryam SahebariMasoud Pezeshki Rad
Nov 8, 2012·International Journal of Endocrinology·Donatella CapalboMariacarolina Salerno
Oct 20, 2009·The Journal of Clinical Endocrinology and Metabolism·E Helen KempAnthony P Weetman
Nov 5, 2008·Internal Medicine·Lorraine Pelletier-MorelEtienne Larger
May 18, 2007·Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·Itsuro Endo, Toshio Matsumoto
May 18, 2007·Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·Masaaki Inaba, Yoshiki Nishizawa
Jul 3, 2013·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Elizabeth KuoChou-Long Huang
Apr 17, 2012·Clinical Biochemistry·Bryan K WardThomas Ratajczak
Apr 28, 2006·Annales d'endocrinologie·C Betterle
Nov 26, 2010·Rheumatic Diseases Clinics of North America·Hong Yan WenLi Yun Zhang
Jun 13, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Anne CharriéNicole Fabien
Mar 31, 2009·Endocrinology and Metabolism Clinics of North America·Catherine J Owen, Tim D Cheetham
Mar 31, 2009·Endocrinology and Metabolism Clinics of North America·Edward M Brown
May 14, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Indra Ramasamy
Sep 27, 2003·Clinical Endocrinology·John A Sayer, Simon H S Pearce
Mar 22, 2012·Clinical Endocrinology·Takafumi NozakiToshiro Hara
Aug 4, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·John P BilezikianJames Sanders
Jul 8, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·E Helen KempAnthony P Weetman
Aug 22, 2006·Molecular Genetics and Metabolism·Naibedya Chattopadhyay, Edward M Brown
Jun 28, 2005·Lancet·Simon J Steddon, John Cunningham
Apr 17, 2015·Clinical Endocrinology·Earn H GanSimon H S Pearce
Jan 29, 2013·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Ilana ParkesYoel Shufaro
Apr 29, 2015·Annales d'endocrinologie·Anthony P Weetman
Jan 16, 2014·Autoimmunity Reviews·Corrado BetterleFabio Presotto
Nov 23, 2006·Arquivos brasileiros de endocrinologia e metabologia·Lília D'Souza-Li

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