Autoantibodies to the GM2-1 islet ganglioside and to GAD-65 at type 1 diabetes onset

Journal of Autoimmunity
F DottaU Di Mario

Abstract

The GM2-1 islet ganglioside has been sequenced, found to be a novel ganglioside structure with a sialic acid moiety in the terminal position and two residues of non-acetylated galactosamine and also shown to be a target of autoantibodies in a subset of ICA+ relatives of type 1 diabetic patients who subsequently progressed to the overt disease. In the present study we determined whether antibodies to GM2-1 or to other pancreatic gangliosides (a) are also expressed at disease onset and (b) are correlated with other diabetes-associated autoantibodies. Pancreatic gangliosides were extracted from human pancreas and purified by thin layer chromatography (TLC). Anti-ganglioside autoantibodies were determined using an indirect immunoperoxidase technique performed directly on TLC plates in the following groups of patients: (a) newly diagnosed type 1 diabetic subjects before insulin therapy (n = 45); all were tested for GAD65 autoantibodies in a fluid-phase RIA using 35S-methionine-labelled recombinant human GAD65. Of these patients, 24 were also tested for insulin autoantibodies (IAA) by a competitive fluid phase radioimmunoassay and 21 were tested for GAD67 reactivity. (b) Forty-two age- and sex-matched normal control subjects. Autoant...Continue Reading

Citations

Mar 5, 2003·Diabetes Technology & Therapeutics·William E WinterDesmond Schatz
Jan 1, 2012·The Review of Diabetic Studies : RDS·Yulan Jin, Jin-Xiong She
Sep 22, 2009·Muscle & Nerve·Marta LucchettaChiara Briani
May 17, 2005·Diabetes/metabolism Research and Reviews·Bernd FrankeTerry J Wilkin
Dec 4, 2010·Clinical Chemistry·William E Winter, Desmond A Schatz
Apr 20, 2018·Diabetologia·Laurits J HolmKarsten Buschard
Nov 8, 2020·Annual Review of Physiology·Bhagirath Chaurasia, Scott A Summers

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