Autoantibody detection in bullous pemphigoid: clinical evaluation of the EUROPLUS™ Dermatology Mosaic

Journal of Immunological Methods
Jan DamoiseauxJan Willem Cohen Tervaert

Abstract

Bullous pemphigoid (BP) is an autoimmune blistering skin disease. Autoantibodies to BP180 and BP230 can be detected by indirect immunofluorescence (IIF) on different substrates (oesophagus, salt-split-skin, BP180-antigen dots, BP230-transfected cells) and ELISA. Here, we compared test characteristics of these test systems. We analysed sera from BP patients (n=60) in whom the clinical diagnosis had been confirmed histopathologically. The control cohort comprised sera from patients with other autoimmune-associated (n=22) or inflammatory (n=35) skin diseases. All samples were tested by IIF (EUROIMMUN™ Dermatology Mosaic) and ELISA (EUROIMMUN and MBL). Anti-BP180 is best detected with BP180-antigen dots by IIF (sensitivity: 88%; specificity: 97%). As compared to IIF, the differences with both BP180 ELISA techniques are small though. Likelihood ratios (LRs) for positive and negative test results are >10 and between 0.1 and 0.2, respectively, for all test systems. Detection of anti-BP230 is highly variable (sensitivity range 38-60%; specificity range 83-98%). Only the IIF test reveals a LR for positive test results >10. Since the LRs for a negative test are all ~0.5, negative test results for anti-BP230 antibodies do not help to excl...Continue Reading

References

Nov 22, 2002·Journal of Dermatological Science·Masakazu KobayashiTakeji Nishikawa
Jun 6, 2003·Best Practice & Research. Clinical Rheumatology·Pieter van PaassenJan Willem Cohen Tervaert
Oct 15, 2003·Arthritis and Rheumatism·Solbritt Rantapää-DahlqvistWalther J van Venrooij
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Sep 18, 2009·Annals of the New York Academy of Sciences·Marilina TampoiaFrancesca Di Serio
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Aug 18, 2010·Autoimmunity Reviews·Enno Schmidt, Detlef Zillikens
Jun 7, 2011·Autoimmunity Reviews·Michael Sticherling, Cornelia Erfurt-Berge

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Citations

Dec 12, 2012·Clinical & Developmental Immunology·Aikaterini PatsatsiDimitrios Sotiriadis
Nov 28, 2013·Scientifica·Jan Damoiseaux
Jul 2, 2019·Journal of the European Academy of Dermatology and Venereology : JEADV·A YangD F Murrell
Jun 19, 2019·The American Journal of Dermatopathology·Rajalakshmi Tirumalae, Inchara Yeliur Kalegowda
Feb 20, 2016·Archives of Dermatological Research·Angelo Valerio MarzanoMauro Alaibac
Sep 26, 2019·Frontiers in Immunology·Sandra SaschenbreckerWolfgang Schlumberger
Aug 30, 2018·Frontiers in Medicine·Pawel PietkiewiczMarian Dmochowski
Feb 1, 2020·Journal of the American Academy of Dermatology·Nina van BeekKristin Rentzsch

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