Autoantibody signatures defined by serological proteome analysis in sera from patients with cholangiocarcinoma

Journal of Translational Medicine
Mohammad Zahid MustafaEric Ballot

Abstract

The challenging diagnosis and poor prognosis of cholangiocarcinoma require the determination of biomarkers. Autoantibodies could be used in the clinic as diagnostic markers for the early detection of tumours. By proteomic approaches, several autoantibodies were proposed as potential markers. We tried in this study, to perform a serological proteome analysis, using various antigenic substrates, including tumours and human liver. Sera from patients (n = 13) and healthy donors (n = 10) were probed on immunoblots performed using 2-dimensionally separated proteins from cholangiocarcinoma cell lines (CCLP1 and CCSW1), from the liver of healthy subject and interestingly, from tumour and adjacent non-tumour liver tissues from five patients with cholangiocarcinoma and tested with their corresponding serum. Spots of interest were identified using mass spectrometry and classified according gene ontology analysis. A comparison of the whole immunoblotting patterns given by cholangiocarcinoma sera against those obtained with normal control sera enabled the definition of 862 spots. Forty-five different proteins were further analysed, corresponding to (1) spots stained with more than four of 13 (30 %) sera tested with the CCLP1 or the CCSW1 ce...Continue Reading

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Citations

May 28, 2017·Scientific Reports·Katherine V GatesLeigh G Griffiths
Mar 7, 2020·Clinical & Translational Immunology·Vanessa M BeutgenFranz H Grus
Jan 12, 2020·Seminars in Immunology·Makoto KobayashiSamir M Hanash

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Methods Mentioned

BETA
electrophoresis
protein array

Software Mentioned

Proteome Discoverer
Adobe Photoshop
Discoverer Proteome

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