Autocrine and paracrine actions of breast tumor aromatase. A three-dimensional cell culture study involving aromatase transfected MCF-7 and T-47D cells

The Journal of Steroid Biochemistry and Molecular Biology
X Z SunS Chen

Abstract

Stable aromatase-expressing MCF-7 and T-47D cell lines (i.e. MCF-7aro and T-47Daro) have been prepared by aromatase cDNA transfection and G418 (neomycin) selection. MCF-7aro was further subjected to a clonal purification. Aromatase activity in the transfected MCF-7 and T-47D cell lines was determined to be 73 +/- 6 pmol/mg/h and 48 +/- 4 pmol/mg/h, respectively. It is thought that these cell lines express aromatase in a stable manner, as demonstrated by a steady expression of the enzyme during culture in the absence of G418. The growth of these cells could be stimulated by androgens (1-10 nM) as demonstrated through a spheroid culture method. The androgen-stimulated growth could be suppressed by 4-hydroxyandrostenedione (4-OHA) (0.01-0.1 mM) or tamoxifen (50 nM-1 microM). In order to test the hypothesis that tumor aromatase can affect breast tumor growth in a paracrine manner, we have carried out cell culture experiments by co-culturing MCF-7 cells with either MCF-7aro or T-47Daro cells. Testosterone (1 nM) increased cell growth to a similar degree for MCF-7/MCF-7aro co-culture (0.75 x 10(6) cells each type) as with MCF-7aro only (2- to 3-fold). In addition, the enzyme activities remained unchanged for MCF-7/MCF-7aro co-culture...Continue Reading

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Citations

Jul 12, 2013·Apoptosis : an International Journal on Programmed Cell Death·Cristina AmaralNatércia Teixeira
Aug 5, 2008·Breast Cancer Research and Treatment·Selma MasriShiuan Chen
Jan 8, 2010·Breast Cancer Research and Treatment·Selma MasriShiuan Chen
Feb 22, 2012·Cell Biochemistry and Biophysics·Mohamed-Elamir F HegazyPaul W Paré
Jun 16, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Shiuan ChenDujin Zhou
Mar 26, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Shiuan ChenChun Yang
Nov 19, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Shiuan ChenElizabeth T Eng
Aug 25, 2001·Breast Cancer Research and Treatment·E T EngS Chen
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shiuan ChenDean Evans
Feb 22, 2011·Omics : a Journal of Integrative Biology·Shiuan Chen
Aug 23, 2012·PloS One·Cristina AmaralNatércia Teixeira
Feb 6, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Shiuan ChenChristina Teng
Jul 13, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Yasuhiro MikiHironobu Sasano
Jul 6, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Shiuan ChenXiwei Wu
Oct 24, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Shiuan ChenXiwei Wu
Mar 8, 2005·The Journal of Steroid Biochemistry and Molecular Biology·J Shields-BotellaJ R Pasqualini
Jan 18, 2006·The Journal of Steroid Biochemistry and Molecular Biology·J R Pasqualini, G S Chetrite
Jan 16, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Cristina AmaralNatércia Teixeira
Sep 11, 2004·The Journal of Biological Chemistry·Shiuan ChenYate-Ching Yuan
Apr 14, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yun WangLai K Leung
Jan 31, 2007·Annals of the New York Academy of Sciences·Yanyan Hong, Shiuan Chen
Jan 1, 2010·Hormone Molecular Biology and Clinical Investigation·Gérard S Chetrite, Jorge R Pasqualini
Dec 1, 2010·Hormone Molecular Biology and Clinical Investigation·Gérard S Chetrite, Jorge R Pasqualini
Apr 7, 2018·Breast Cancer Research and Treatment·Karineh PetrossianShiuan Chen
Apr 16, 2005·Molecular Cancer Research : MCR·Toru ItohShiuan Chen

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