Autocrine expression of hepatocyte growth factor and its cytoprotective effect on hepatocyte poisoning

World Journal of Gastroenterology : WJG
Yong HeHai-Min Li

Abstract

To construct pEGFP-hepatocyte growth factor (HGF) expression vector, the to detect its expression in transfected human hepatocytes, and to investigate the influence of autocrine HGF expression on the proliferative potential and cytoprotective effects in human hepatocytes. Human HGF cDNA was ligated to the pEGFP vector. Recombinant plasmid was transfected into human hepatocyte line QZG with liposome. Expression of HGF protein was observed by fluorescence microscopy and immunohistochemistry. Hepatic cells were collected 24, 48, and 72 h after transfection to detect the number of ((3)H)-TdR uptake in DNA. DNA synthesis was observed by using PCNA stain immunohistochemistry. Acute liver cell damage was induced by carbon tetrachloride. Cytoprotective effect was observed by examining the survival rate of hepatocytes and leakage of intracellular alanine transaminase (ALT) and potassium ions. HGF identification of pEGFP-HGF by enzyme digestion showed that HGF fragment was cloned into BamH I and Sal I sites of pEGFP-N3. Expression of GFP in transfected hepatocytes was observed with fluorescence microscopy. The ((3)H)-TdR uptake became 7 times as many as in the control group 96 h after transfection. After HGF transfection, the survival ra...Continue Reading

References

Feb 4, 1999·Nature Medicine·T UekiJ Fujimoto
Jun 29, 1999·The Journal of Surgical Research·H KondoM Kodama
Jul 1, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K KosaiT Nakamura
Jan 31, 2002·World Journal of Gastroenterology : WJG·Wei Xu, Shu-Guang Wu
Mar 28, 2002·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Jacek Borawski, Michak Myśliwiec
Nov 6, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Junko HataSatoshi Asano
Dec 17, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hiroshi Funakoshi, Toshikazu Nakamura
Jan 10, 2003·Journal of Cellular Biochemistry·Yu-Wen Zhang, George F Vande Woude
Mar 12, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shishiro OeYasuhiko Tabata
Jun 26, 2003·Methods in Molecular Biology·Geoffrey S Waldo
Jul 23, 2003·Genesis : the Journal of Genetics and Development·Katrina L ScarffPhillip I Bird
Oct 11, 2003·Molecular and Cellular Neurosciences·Jouji KokuzawaNoboru Sakai

❮ Previous
Next ❯

Citations

May 15, 2007·Liver International : Official Journal of the International Association for the Study of the Liver·Fu-Sheng WuWei Wu
Apr 22, 2006·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·F WuW Zhao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nan fang yi ke da xue xue bao = Journal of Southern Medical University
Shu-Ting RenGuang-dao Gao
Nihon rinsho. Japanese journal of clinical medicine
Y Nakamura, H Makino
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
M Tamura, Y Daikuhara
Nihon rinsho. Japanese journal of clinical medicine
K SugimotoT Nakamura
© 2021 Meta ULC. All rights reserved