Autocrine IL-6 regulates GRO-alpha production in thymic epithelial cells

Cytokine
Yau-Lin TsengChiou-Feng Lin

Abstract

Thymic interleukin-6 (IL-6), which is produced primarily by thymic epithelial cells, is an important cytokine for T cell growth and differentiation in the thymus. In the present study, we investigated the effects of autocrine IL-6 on the cytokines in primary cultures of human thymic epithelial cells. Using an antibody array and ELISA, in addition to IL-8 and MCP-1, growth-related oncogene-alpha (GRO-alpha) was subsequently expressed after IL-6 production. Either IL-6 or GRO-alpha was time-dependently and strongly expressed in medium with and without serum. Neutralizing IL-6 significantly reduced GRO-alpha production. Inhibiting Janus kinase 2 (Jak2) spontaneously blocked GRO-alpha production with or without IL-6 stimulation. We conclude that primary human thymic epithelial cells produce GRO-alpha and that its expression is regulated primarily by autocrine IL-6 and IL-6-activated Jak2 signaling.

Citations

Jan 5, 2013·The British Journal of Nutrition·Ivanna Novotny NúñezGabriela Perdigón
Feb 1, 2011·International Journal of Biological Sciences·Hsiu-Hsia LinSin-Tak Chu
Jan 13, 2011·The Journal of Physiology·Line PedersenPernille Hojman
Apr 5, 2012·Nature Reviews. Endocrinology·Bente K Pedersen, Mark A Febbraio
Aug 7, 2013·Experimental Biology and Medicine·Nevena Arsenović-RaninGordana Leposavić

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