Autocrine production of biologically active hepatocyte growth factor (HGF) by injured human skin

Journal of Dermatological Science
F NayeriUno Carlsson

Abstract

Hepatocyte growth factor (HGF) is a potent regenerative factor involved in wound healing. Previous studies have shown that mesenchymal cells produce HGF, stimulating epithelial cells in a paracrine fashion. To examine whether autocrine HGF production by keratinocytes can occur upon skin injury. A 31-year-old male patient sustained a burn affecting 80% of his total body surface area. Biopsies were taken from intact skin near the injured area, and skin keratinocytes were separated and cultured. Conditioned medium from keratinocytes was analyzed for HGF by ELISA, surface plasmon resonance (SPR), and dot blotting. Binding of HGF from conditioned medium to its receptor, c-Met, was compared with recombinant HGF by SPR. Finally, we examined the motogenic effect on mouse transformed skin epithelial cells (CCL-53.1) of HGF from conditioned medium. HGF was detected in the cultured keratinocyte medium. Similar to recombinant HGF, HGF from conditioned medium had a high affinity for dextran sulfate and albumin, and the same epitopes were engaged by the interaction of HGF with the c-Met receptor. The conditioned medium from keratinocytes obtained from the burn patient, but not medium from keratinocytes obtained from healthy volunteers, accel...Continue Reading

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Citations

Oct 13, 2010·The Journal of Biological Chemistry·Kyle E LandgrafRobert A Lazarus
Apr 19, 2013·Journal of Periodontology·Johanna LönnNils Ravald
Jun 17, 2014·The Journal of Investigative Dermatology·Joanna Nikitorowicz-BuniakRichard Stratton
Jul 22, 2010·Molecular Carcinogenesis·Okkyung RhoJohn Digiovanni
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Jul 18, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jea-Hyun BaekThomas Hieronymus
Jul 2, 2020·AIMS Neuroscience·Zygmunt SiedleckiMaciej Śniegocki
Jun 19, 2021·Angewandte Chemie·Momoko AkiyamaShinsuke Sando

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