PMID: 8594307Jan 1, 1996Paper

Autocrine regulation of erythropoietin gene expression in human hepatocellular carcinoma cells

Life Sciences
T OhigashiJ W Fisher

Abstract

We have previously reported that a marked increase in erythropoietin (Epo) production can be demonstrated in Hep3B cell cultures in response to hypoxia (1). In order to determine whether this increase involves an autocrine mechanism, we have investigated the effects of purified human recombinant Epo (rHuEpo) on Epo production. Purified rHuEpo (5-80 mU/ml) produced a significant increase above control levels of Epo in Hep3B cell cultures under normoxic (20% O2) conditions. Hypoxic (1% o2) incubation of Hep3B cells with rHuEpo caused an increase over control levels of EpomRNA. Hep3B cells also expressed Epo receptor (Epo-R) transcripts. Binding studies [125I]Epo revealed that Hep3B cells contain a single class of binding site (kd=2.9 nmol/L and Bmax=1760 sites/cell). Antierythropoietin receptor monoclonal antibody inhibited the rHuEpo induced elevation in medium levels of Epo and blocked [125I]-Epo binding to Hep3B cell membranes. These results demonstrate that the expression of EpomRNA may be controlled, at least in part, by an autocrine mechanism.

References

Jun 1, 1991·The Journal of Clinical Investigation·S SuemoriD K Podolsky
Apr 30, 1990·Biochemical and Biophysical Research Communications·M Mason-GarciaB S Beckman
Oct 1, 1988·Journal of Cellular Physiology·M J Koury, M C Bondurant
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M A GoldbergH F Bunn
Nov 1, 1988·Journal of Cellular Physiology·K SawadaC I Civin
May 15, 1983·The Biochemical Journal·J C CalvoE H Charreau
Feb 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mark J MillerIan Parker
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER
Jan 25, 2012·Obstetrics and Gynecology·Yao DingSylvia H Yeh

❮ Previous
Next ❯

Citations

Dec 18, 2013·Annals of Hematology·Steve ElliottWolfgang Jelkmann
Aug 1, 2012·Biologics : Targets & Therapy·Steve Elliott, Angus M Sinclair
Oct 8, 2004·Internal Medicine·Wolfgang Jelkmann
Apr 7, 2007·Journal of Neurosurgery·Ahmed MohyeldinAjay Verma
Jun 22, 2005·Neoplasia : an International Journal for Oncology Research·Ahmed MohyeldinAjay Verma
Nov 23, 2000·Journal of Surgical Oncology·M MatsuyamaT Inoue
Aug 24, 2004·Annals of Hematology·Wolfgang Jelkmann, Klaus Wagner
Aug 1, 2000·Kidney International·C Westenfelder, R L Baranowski
Dec 8, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Anders OsterborgWolfgang Jelkmann
Apr 25, 2008·Critical Reviews in Oncology/hematology·Wolfgang JelkmannArthur J Sytkowski

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

© 2021 Meta ULC. All rights reserved