Autocrine Semaphorin3A stimulates eukaryotic initiation factor 4E-dependent RhoA translation in breast tumor cells.

Experimental Cell Research
Hongjie Pan, Robin E Bachelder

Abstract

Translation of the small G protein RhoA in neurons is regulated by the eukaryotic translation initiation factor eIF4E. Here we show that this translation factor also regulates RhoA expression and activity in breast cancer cells. The introduction of eIF4E into breast tumor cells increased RhoA protein levels, while expression of an eIF4E siRNA reduced RhoA expression. Previous studies indicate that the axon repulsion factor Semaphorin3A (Sema3A) stimulates the eIF4E-dependent translation of RhoA in neurons, and breast tumor cells support autocrine Sema3A signaling. Accordingly, we next examined if autocrine Sema3A signaling drives eIF4E-dependent RhoA translation in breast cancer cells. The incubation of breast tumor cells with recombinant Sema3A rapidly increased eIF4E activity, RhoA protein levels, and RhoA activity. This Sema3A activity was blocked in tumor cells expressing an shRNA-specific for the Sema3A receptor, Neuropilin-1 (NP-1), as well as in cells incubated with an eIF4E inhibitor. Importantly, RhoA protein levels were reduced in Sema3A shRNA-expressing compared to control shRNA-expressing breast tumor cells, demonstrating that autocrine Sema3A increases RhoA expression in breast cancer. Considering that Sema3A suppr...Continue Reading

References

Feb 20, 1995·International Journal of Cancer. Journal International Du Cancer·V KerekatteA De Benedetti
Aug 22, 1997·Cell·A L KolodkinD D Ginty
Jan 28, 1998·Oncogene·L del PesoJ C Lacal
Jun 27, 1998·The Journal of Biological Chemistry·S S Kantak, R H Kramer
Apr 27, 1999·The International Journal of Biochemistry & Cell Biology·A De Benedetti, A L Harris
Oct 3, 1999·Microscopy Research and Technique·P DefilippiG Tarone
Oct 16, 1999·Cell·T TakahashiS M Strittmatter
Jun 30, 2000·Current Biology : CB·W T ArthurK Burridge
Aug 17, 2002·The Journal of Biological Chemistry·Valerie PerrotJ Silvio Gutkind
Aug 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jennifer AurandtKun-Liang Guan
Apr 18, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mari KaarbøWayne G Murrell
Mar 17, 2004·Seminars in Cancer Biology·Arthur M MercurioJun Chung
Apr 20, 2004·Oncogene·Arrigo De Benedetti, Jeremy R Graff
Dec 3, 2004·Cancer Research·Kaylene J SimpsonArthur M Mercurio
Aug 19, 2005·Nature·Karen Y WuSamie R Jaffrey
Sep 13, 2008·Breast Cancer Research and Treatment·Hongjie PanRobin E Bachelder

❮ Previous
Next ❯

Citations

Feb 24, 2012·Molecular Biology of the Cell·Lei XingGary J Bassell
Feb 21, 2013·Experimental Cell Research·Chenghua Gu, Enrico Giraudo
Dec 4, 2012·Journal of Internal Medicine·G SeriniE Giraudo
Jul 28, 2012·Expert Opinion on Therapeutic Targets·Yoshio GoshimaFumio Nakamura
Jul 22, 2014·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Lucile Astorgues-XerriEric Raymond
May 4, 2018·Scientific Reports·Daniëlle de RidderAymen I Idris
Jul 28, 2019·Biochemical and Biophysical Research Communications·Zhili HeJonathan A Raper

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Tumorigenesis

Breast tumorigenesis involves the production or formation of tumor(s) in breast tissue. Discover the latest research on breast tumorigenesis here.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Axon Guidance

Axon guidance is a complex neural developmental field that investigates mechanisms through which neurons send out axons to reach its target. Here is the latest research in this domain.