Feb 1, 1976

Autodegradation of rat liver polyribosomes in vitro

A A Del'vig, S S Debov


In vihro decay of rat liver polyribosomes is studied under conditions of occuring phosphorolytic activity of polynucleotidephosphorylase (PNPase) and, in contrast, excluding PNPase activity (in the absence of orthophosphate). The rate of polyribosome degradation is found to be higher in the presence of orthophosphate, than in its absence. Sedimentation coefficient of ribosomes formed from degraded polyribosomes in the absence of orthophosphate is 70S, while that of ribosomes formed under phosphorolysis is 67S. It is suggested that polyribosome degradation in the presence of orthophosphate is carried out by the combined action of 5'-RNase and PNPase. 5'-RNase degrades mRNA and rRNA without disturbing the integrity of ribosomal complexes, while PNPase phosphorolyzes the fragments formed from the 3'-OH terminal, successively releasing ribosomes. The rate of polysome inactivation in the presence of orthophosphate (half-life period being approximately 17 min) is considerably higher than that in the absence of orthophosphate (half-life period being 37 min). The amount of active polysomes in samples incubated for 3 hours both in the presence and in the absence of orthophosphate is 16% and 24% of the total amount of active polysomes, r...Continue Reading

  • References
  • Citations


  • We're still populating references for this paper, please check back later.
  • References
  • Citations


  • This paper may not have been cited yet.

Mentioned in this Paper

Set of Polysomal Ribosomes
Complex (molecular entity)
PNPT1 gene
Ribosomal RNA
Alkaline Ribonuclease
Pancreatic ribonuclease
Sedimentation Procedure

About this Paper

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

LRRK2 & Immunity During Infection

Mutations in the LRRK2 gene are a risk-factor for developing Parkinson’s disease. However, LRRK2 has been shown to function as a central regulator of vesicular trafficking, infection, immunity, and inflammation. Here is the latest research on the role of this kinase on immunity during infection.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Meningococcal Myelitis

Meningococcal myelitis is characterized by inflammation and myelin damage to the meninges and spinal cord. Discover the latest research on meningococcal myelitis here.

Alzheimer's Disease: MS4A

Variants within membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease by recent genome-wide association studies. Here is the latest research.