Autodisplay of enzymes--molecular basis and perspectives

Journal of Biotechnology
Joachim JoseMark George Teese

Abstract

To display an enzyme on the surface of a living cell is an important step forward towards a broader use of biocatalysts. Enzymes immobilized on surfaces appeared to be more stable compared to free molecules. It is possible by standard techniques to let the bacterial cell (e.g. Escherichia coli) decorate its surface with the enzyme and produce it on high amounts with a minimum of costs and equipment. Moreover, these cells can be recovered and reused in several subsequent process cycles. Among other systems, autodisplay has some extra features that could overcome limitations in the industrial applications of enzymes. One major advantage of autodisplay is the motility of the anchoring domain. Enzyme subunits exposed at the cell surface having affinity to each other will spontaneously form dimers or multimers. Using autodisplay enzymes with prosthetic groups can be displayed, expanding the application of surface display to the industrial important P450 enzymes. Finally, up to 10⁵-10⁶ enzyme molecules can be displayed on a single cell. In the present review, we summarize recent achievements in the autodisplay of enzymes with particular attention to industrial needs and process development. Applications that will provide sustainable ...Continue Reading

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Citations

Mar 13, 2013·Journal of Industrial Microbiology & Biotechnology·Iván Muñoz-Gutiérrez, Alfredo Martinez
Aug 12, 2014·Applied Microbiology and Biotechnology·Jan SchüürmannJoachim Jose
Aug 17, 2014·Journal of Biotechnology·Iasson E P TozakidisJoachim Jose
Jan 21, 2016·Journal of Pharmaceutical and Biomedical Analysis·Andre BollackeJoachim Jose
Jun 14, 2015·Biotechnology Advances·Tsutomu Tanaka, Akihiko Kondo
Nov 20, 2015·Nature Protocols·Richard N Besingi, Patricia L Clark
Jan 13, 2015·New Biotechnology·Iasson E P TozakidisJoachim Jose
Nov 26, 2013·Biochimica Et Biophysica Acta·Peter van UlsenJoen Luirink
Jul 17, 2013·Critical Reviews in Microbiology·Toon NicolayStijn Spaepen
Sep 26, 2014·Extremophiles : Life Under Extreme Conditions·L E PetrovskayaM P Kirpichnikov
Sep 15, 2016·PloS One·Esther BraselmannPatricia L Clark
Oct 27, 2016·Scientific Reports·Martin GustavssonGen Larsson
Sep 15, 2018·Chembiochem : a European Journal of Chemical Biology·Shubhendu PaleiHenning D Mootz
May 3, 2019·Microbial Biotechnology·Iasson E P TozakidisJoachim Jose
Nov 23, 2019·Angewandte Chemie·Benedikt JedlitzkeHenning D Mootz
Aug 10, 2013·Biological Chemistry·Iris GawarzewskiJoachim Jose
Aug 6, 2021·Applied Biochemistry and Biotechnology·Lada E PetrovskayaMikhail P Kirpichnikov
Apr 29, 2015·Industrial & Engineering Chemistry Research·Mason R SmithFei Wen

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