Autogenous and global control of the flagellar master operon, flhD, in Salmonella typhimurium

Molecular & General Genetics : MGG
Kazuhiro Kutsukake

Abstract

Expression of the flagellar master operon, flhD, is known to be affected by growth conditions and by mutations in a variety of genes. In the present work, the transcriptional control of the Salmonella typhimurium flhD operon was investigated in various genetic backgrounds. First, we examined the effect of mutations in the global regulators cAMP-CRP, H-NS, OmpR and RpoS. Mutations in the cya, crp or hns gene reduced but did not eliminate flhD expression. However, expression was completely inhibited in the cya hns and crp hns double mutants. These results indicate that cAMP-CRP and H-NS independently activate the flhD operon and that maximal expression is attained in the presence of both regulators. On the other hand, the ompR and rpoS mutations did not affect either the motility phenotype or flhD expression. We next examined the expression of a chromosomal flhD-lac fusion gene in the presence of a plasmid carrying the wild-type flhD operon. It was found that under this condition the chromosomal flhD operon was repressed or activated, depending on the intracellular activity of FliA, an alternative sigma factor specific for late flagellar operons. In the absence of FliA or in the presence of both FliA and its cognate anti-sigma fa...Continue Reading

Citations

Sep 11, 2010·Antonie van Leeuwenhoek·Adrianna RaczkowskaKatarzyna Brzostek
May 17, 2008·Nature Reviews. Microbiology·Fabienne F V Chevance, Kelly T Hughes
Jan 20, 1999·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·S I Aizawa, T Kubori
Nov 25, 2003·Nucleic Acids Research·Ruy JáureguiEnrique Merino
Apr 12, 2012·Applied and Environmental Microbiology·Matthew D MastropaoloStuart B Levy
Dec 31, 2008·Journal of Bacteriology·Christopher E WozniakKelly T Hughes
Nov 6, 2007·Journal of Bacteriology·María E CastelliEleonora García Véscovi
May 13, 2008·Journal of Bacteriology·Supreet SainiChristopher V Rao
Jul 26, 2011·Journal of Bacteriology·Markus WilhelmsSusana Merino
Jun 13, 2000·Journal of Bacteriology·Y K Kim, L L McCarter
Jan 16, 2002·Journal of Bacteriology·Toshifumi TomoyasuTomoko Yamamoto
Jul 18, 2003·Journal of Bacteriology·Bonnie J Stewart, Linda L McCarter
Dec 17, 2004·Journal of Bacteriology·Lisa M MaurerJoan L Slonczewski
Mar 4, 2005·Journal of Bacteriology·Therese M O'SheaKaren L Visick
Mar 4, 2006·Journal of Bacteriology·Jonathan FryeKelly T Hughes
Apr 20, 2006·Journal of Bacteriology·Hee Jung Lee, Kelly T Hughes
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·G S Chilcott, K T Hughes
Aug 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·L L McCarter
Dec 26, 2002·Annual Review of Biochemistry·Howard C Berg
Jan 19, 1999·Annual Review of Microbiology·K T Hughes, K Mathee
Jan 29, 2000·Genes & Genetic Systems·T IkebeK Kutsukake
May 3, 2000·Genes & Genetic Systems·K KutsukakeS Yamamoto
Sep 12, 2002·Biological Chemistry·Oliver Schröder, Rolf Wagner

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