PMID: 2500421Jul 1, 1989Paper

Autogenous regulation of the Escherichia coli ksgA gene at the level of translation

Journal of Bacteriology
B van GemenP H van Knippenberg

Abstract

Various plasmids that contain the Escherichia coli ksgA gene, which encodes a 16S rRNA adenosine dimethyltransferase (methylase), were constructed. In one of these plasmids, the DNA encoding the N-terminal part of the methylase was fused to the lacZ gene, and in another construct, the ksgA gene contained a deletion which resulted in a truncated version of the methylase. When a cell contained one plasmid directing the synthesis of the intact, active methylase and another plasmid encoding the methylase-beta-galactosidase protein, production of the latter product became strongly reduced. Likewise, synthesis of the truncated version of the methylase was diminished when the cell at the same time contained a plasmid producing the complete enzyme. These results were partly substantiated by in vitro experiments with a coupled transcription-translation assay system. By using a recently developed gel electrophoresis system for measuring protein-nucleic acid interactions, a specific binding of the ksgA methylase with its own mRNA could be established. Our results demonstrate that the expression of the ksgA gene can be, at least partly, autogenously controlled at the level of translation.

References

Nov 1, 1987·Molecular & General Genetics : MGG·K R Kearney, M Nomura
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M SpringerM Grunberg-Manago
Nov 11, 1987·Nucleic Acids Research·J F MilliganO C Uhlenbeck
Jan 1, 1983·Progress in Nucleic Acid Research and Molecular Biology·L BoschJ M Van Noort

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Citations

Nov 8, 2008·Molecular Microbiology·Keith ConnollyGloria Culver
Feb 7, 2012·The Journal of Biological Chemistry·Daniel BoehringerNenad Ban
Feb 15, 2002·Journal of Bacteriology·Andrew J PeaseMalcolm E Winkler
Sep 1, 1996·Journal of Bacteriology·M KojicB Vasiljevic
Apr 1, 2021·EcoSal Plus·Angela TramontiRoberto Contestabile
May 12, 2004·Journal of Molecular Biology·Heather C O'FarrellJason P Rife
Dec 1, 1993·Microbiological Reviews·M Riley
Apr 20, 2006·Archives of Biochemistry and Biophysics·Pooja M Desai, Jason P Rife

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