Autographa californica nuclear polyhedrosis virus: subcellular localization and protein trafficking of BV/ODV-E26 to intranuclear membranes and viral envelopes

Virology
H BeniyaM D Summers

Abstract

The Autographa californica nuclear polyhedrosis virus da26 gene codes for an envelope protein of both budded virus (BV) and occlusion derived virus (ODV). Western blot and temporal analysis of infected cell extracts detected a protein of 26 kDa by 4 h postinfection (p.i.). The amount of protein increased by 16 h p.i. and remained at high levels throughout infection. By 36 h p.i. several additional immunoreactive proteins were detected which migrated at approximately 18 kDa and remained through 96 h p.i. Western blot analysis of purified virus envelope and nucleocapsid preparations revealed that both the 26- and 18-kDa proteins are structural proteins of the envelope of BV and ODV. Immunoelectron microscopy performed at a time when only the 26-kDa species of the protein was present confirmed that the protein located to ODV envelope. The protein was named BV/ODV-E26 to designate incorporation into viral progeny, envelope location, and apparent molecular weight. Studies designed to follow localization of BV/ODV-E26 demonstrated that early in infection, the protein was incorporated into cytoplasmic vesicles and by 16 h p.i., BV/ODV-E26 was detected in the nucleus associated with virus-induced intranuclear microvesicles and ODV enve...Continue Reading

References

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Citations

May 7, 2010·Journal of Virology·Ranran WangZhihong Hu
May 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sharon C BraunagelMax D Summers
Aug 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·S C BraunagelM D Summers
Mar 23, 2011·Biotechnology Progress·Lilí E Gallo-RamírezLaura A Palomares
Jan 25, 2007·Journal of Biochemistry and Molecular Biology·Dun Wang, Chuan-Xi Zhang
May 23, 2006·Nature Structural & Molecular Biology·Suraj SaksenaSharon C Braunagel
Oct 18, 2005·The Journal of General Virology·WonKyung KangShogo Matsumoto
Oct 12, 2012·Molecular Biology Reports·Yong LiuWenbing Wang

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