Autographic assay for the rapid detection of antioxidant capacity of liquid and semi-solid pharmaceutical formulations using ABTS•+ immobilized by gel entrapment.

AAPS PharmSciTech
Iris Catiana ZampiniM I Isla

Abstract

An autographic assay suitable for the detection of antioxidant compounds in a complex matrix (liquid and semi-solid pharmaceutical formulations) or in isolated compounds was described. The pre-formed radical monocation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(•+)) was generated by oxidation of ABTS with potassium persulfate and reduced in the presence of hydrogen-donating antioxidants. For a further comparative estimation of its applicability and sensitivity, different medicinal plant extracts, hydrogels and antioxidant compounds were dot seeded or chromatographed on silica gel (TLC) and revealed with ABTS(•+) solution (System I) or ABTS(•+) immobilized by gel entrapment (System II). Both systems were effective and able to detect antioxidant activity in a micromolar range in seconds. System II was more sensitive and reproducible than System I. This micromethod is quick, inexpensive, and particularly helpful whether it works with numerous samples or on a small scale.

References

Apr 22, 1996·FEBS Letters·N J MillerC A Rice-Evans
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Aug 6, 1998·Bioscience, Biotechnology, and Biochemistry·T YamaguchiJ Terao
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Dec 14, 2004·Journal of Ethnopharmacology·D IvanovaT Yankova
Sep 3, 2005·Journal of Ethnopharmacology·Iris C ZampiniMaria I Isla
Feb 4, 2006·Phytochemical Analysis : PCA·I Ayelen RamalloRicardo L E Furlan

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Citations

Feb 24, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Ana Lilia SalasMaría Inés Isla
Nov 18, 2015·Critical Reviews in Analytical Chemistry·Andrei A BunaciuHassan Y Aboul-Enein
Dec 1, 2017·Anais Brasileiros De Dermatologia·Silas Arandas Monteiro E SilvaGislaine Ricci Leonardi

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