PMID: 2491605Jan 1, 1989Paper

Autoimmunity induced by lactate dehydrogenase-elevating virus: monoclonal autoantibodies against Golgi antigens and other subcellular elements

Autoimmunity
A GrossmannE Weiland

Abstract

Immunocompetent mice infected with LDVAGIA developed autoantibodies against Golgi antigen as early as 6-7 days postinfection preceding anti-viral antibodies for nearly a week. The anti-Golgi antibody titres peaked between two and three weeks postinfection independent of the applied virus dose. Already one week postinfection anti-Golgi autoantibodies were prominent in IgG subclasses IgG2a and b. Spleen cells from these mice were fused with myeloma cells and the culture fluids were screened by indirect immunofluorescence for antibodies reactive with the Golgi antigen of normal cultured cells. A panel of cloned stable antibody-producing hybridomas has been obtained. Some antibodies showed broad cross-species reactivity, recognizing similar antigenic determinants in the Golgi region of mouse, rat and other mammalian cells and also in avian and piscine cells, whereas others recognized determinants in this cell compartment only in mammalian cells and one recognized Golgi antigen only in particular murine tumor cells. From 19 Golgi antibody producing hybridomas 3 secreted IgM-antibodies, 10 synthesized autoantibodies of the subclass IgG2a and 6 of IgG2b. Applied in immunoelectron microscopy mABs decorated the Golgi organelle. A consid...Continue Reading

References

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Citations

May 1, 1996·Journal of Virological Methods·W A CafrunyR F Nelson
Feb 26, 2005·Journal of Virology·Barbara G KluppThomas C Mettenleiter
Feb 29, 2008·Immunopharmacology and Immunotoxicology·Kazuhisa NozawaEdward K L Chan
May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·John E ButlerKelly M Lager
Jan 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Caitlin D LemkeJohn E Butler
Jul 10, 1999·Journal of Virology·E J SnijderA A de Vries
Mar 1, 1994·La Revue de médecine interne·G RenierA Chevailler

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