Autoindicating optical properties of laccase as the base of an optical biosensor film for phenol determination

Analytical and Bioanalytical Chemistry
J SanzJavier Galbán

Abstract

In the context of sustainable analytical chemistry, phenol has been determined through its enzymatic reaction with laccase. The method has been studied and optimized through the autoindicating optical properties of laccase both by intrinsic molecular absorption and fluorescence. The method shows a linear range from 9.79·10(-6) to 7.50·10(-4) M with a relative standard deviation of 1.07 %. The molecular absorption methodology has been implemented in a polyacrylamide film for the design of an autoindicating optical sensor. In order to increase the lifetime of the sensor, the reversibility study of the enzymatic reaction has proposed, as a novelty, the regeneration of laccase with an oxidase-type enzyme (glucose oxidase). The lifetime of the sensor film has improved from 15 to 30 measurements. The reaction mechanism has also been studied and confirmed by fluorescence and molecular absorption. The method leads to the determination of phenol in environmental samples.

References

May 29, 1999·Applied and Environmental Microbiology·K LiK E Eriksson
Sep 24, 1999·Biochemical and Biophysical Research Communications·S D'AuriaJ R Lakowicz
Apr 20, 2001·Luminescence : the Journal of Biological and Chemical Luminescence·J GalbánJ R Castillo
Sep 14, 2001·Biochemical and Biophysical Research Communications·H UchidaT Uwajima
Apr 5, 2003·Current Biology : CB·Patricia A HuntTerry J Hassold
May 2, 2003·Analytical Chemistry·James E NobleAnthony E G Cass
Apr 1, 2006·Trends in Biotechnology·Sergio Riva
Dec 21, 2006·The Analyst·Vanesa SanzJavier Galbán
Feb 6, 2007·Biosensors & Bioelectronics·Vanesa SanzJavier Galbán
Sep 4, 2007·Protein Science : a Publication of the Protein Society·Yaji TianHomme W Hellinga
Apr 15, 2008·Bioresource Technology·Selvia Kurniawati, James A Nicell
Oct 16, 2008·Protein and Peptide Letters·Javier GalbánSusana de Marcos
Jan 15, 2007·Talanta·Dilek OdaciAzmi Telefoncu
Apr 30, 2007·Talanta·Juozas Kulys, Irina Bratkovskaja
Jan 22, 2010·Advances in Biochemical Engineering/biotechnology·Amol DateSylvia Daunert

❮ Previous
Next ❯

Citations

Feb 25, 2014·Analytica Chimica Acta·Liyun ZhangYoung-Tae Chang
Dec 17, 2016·Biotechnology and Applied Biochemistry·Maria Lepore, Marianna Portaccio
Dec 16, 2017·Bioanalysis·Jesús NavarroSusana de Marcos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Hiroaki OtakaMasatoshi Morita
Environmental Science & Technology
Arnold SchecterLinda Birnbaum
© 2021 Meta ULC. All rights reserved