Autoinduction Specificities of the Lantibiotics Subtilin and Nisin

Applied and Environmental Microbiology
Tobias SpießKarl-Dieter Entian

Abstract

The biosynthesis of the lantibiotics subtilin and nisin is regulated by autoinduction via two-component systems. Although subtilin is structurally closely related to nisin and contains the same lanthionine ring structure, both lantibiotics specifically autoinduce their biosynthesis. Subtilin and also the subtilin-like lantibiotics entianin and ericin autoinduce the two-component system SpaRK of Bacillus subtilis, whereas the biosynthesis of nisin is autoinduced via the two-component system NisRK of Lactococcus lactis. Autoinduction is highly specific for the respective lantibiotic and therefore of major importance for the functional expression of genetically engineered subtilin-like lantibiotics. To identify the structural features required for subtilin autoinduction, subtilin-nisin hybrids and specific point mutations of amino acid position 1 were generated. For subtilin autoinduction, the N-terminal tryptophan is the most important for full SpaK activation. The failure of subtilin to autoinduce the histidine kinase NisK mainly depends on the N-terminal tryptophan, as its single exchange to the aliphatic amino acid residues isoleucine, leucine, and valine provided NisK autoinduction. In addition, the production of subtilin var...Continue Reading

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Citations

May 2, 2016·Journal of Dairy Science·Xiaoxuan GeJin Zhong
Apr 7, 2019·Applied and Environmental Microbiology·Christoph GeigerKarl-Dieter Entian
Jan 17, 2020·Expert Opinion on Drug Discovery·Stephanie Kate Sandiford
Mar 1, 2020·Scientific Reports·Enriqueta Garcia-GutierrezPaul D Cotter
Jan 9, 2020·World Journal of Microbiology & Biotechnology·Quan LiuKuide Yin
Jul 16, 2017·Applied and Environmental Microbiology·Christoph GeigerKarl-Dieter Entian
Mar 16, 2019·Frontiers in Microbiology·Simon CaulierJacques Mahillon
Jun 11, 2019·Frontiers in Microbiology·Alexey S Vasilchenko, Eugene A Rogozhin
Jul 14, 2021·Current Microbiology·Basista Rabina SharmaPrakash M Halami
Aug 17, 2021·Frontiers in Bioengineering and Biotechnology·Caiyun LiAiying Li
Aug 7, 2019·ACS Synthetic Biology·Feng LiuQiang Hua

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