Autologous Bone Marrow Mesenchymal Stem Cells Improve the Quality and Stability of Vascularized Flap Surgery of Irradiated Skin in Pigs

Stem Cells Translational Medicine
Christine LinardMarc Benderitter

Abstract

Cutaneous radiation syndrome has severe long-term health consequences. Because it causes an unpredictable course of inflammatory waves, conventional surgical treatment is ineffective and often leads to a fibronecrotic process. Data about the long-term stability of healed wounds, with neither inflammation nor resumption of fibrosis, are lacking. In this study, we investigated the effect of injections of local autologous bone marrow-derived mesenchymal stromal cells (BM-MSCs), combined with plastic surgery for skin necrosis, in a large-animal model. Three months after irradiation overexposure to the rump, minipigs were divided into three groups: one group treated by simple excision of the necrotic tissue, the second by vascularized-flap surgery, and the third by vascularized-flap surgery and local autologous BM-MSC injections. Three additional injections of the BM-MSCs were performed weekly for 3 weeks. The quality of cutaneous wound healing was examined 1 year post-treatment. The necrotic tissue excision induced a pathologic scar characterized by myofibroblasts, excessive collagen-1 deposits, and inadequate vascular density. The vascularized-flap surgery alone was accompanied by inadequate production of extracellular matrix (ECM...Continue Reading

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Citations

Apr 20, 2019·Aesthetic Surgery Journal·Yizuo CaiWei Li
Jul 17, 2019·Journal of Tissue Engineering and Regenerative Medicine·Timo RademakersVanessa L S LaPointe
Jun 6, 2020·European Radiology Experimental·Matheus da Silva SantinJosé Rosa Gomes
Oct 21, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Krisztina NikovicsAnne-Laure Favier

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Methods Mentioned

BETA
surgical resection
biopsies
ELISA
protein assay
PCR
biopsy
scraping

Software Mentioned

Histolab

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