Jun 27, 2007

Autologous buccal keratinocyte implantation for the prevention of stenosis after EMR of the esophagus

Gastrointestinal Endoscopy
Tadashi SakuraiYoshio Hori

Abstract

EMR is a minimally invasive and well-accepted therapy for early esophageal cancer. However, extensive or circumferential EMR induces stricture formation. Cultured skin is now clinically applicable by using the technology of regenerative medicine. Esophageal mucosa also consists of keratinocytes, the same as with skin. We, therefore, hypothesized that, by applying the technology of regenerative medicine, the stricture occurring in the esophagus after EMR on broad lesions could be prevented. The aim of this study was to evaluate, in a swine model, the effect of autologous keratinocyte implantation at the site after EMR to prevent stricture. With the pig under general anesthesia, EMR was carried out by using the cap technique. Two separate areas were resected by EMR. One area was left as a control. For the other area, autologous buccal keratinocytes were injected endoscopically. The outcome, after 2 weeks, was evaluated by endoscopy, macroscopy, and histology. At 2 weeks after EMR, scar formation and stricture were observed in the control lesion. However, in the keratinocyte implanted lesion, the lesion was covered with an epithelium and the luminal surface of the lesion was flat, without ulceration. These results showed the feasi...Continue Reading

Mentioned in this Paper

Buffers
Endoscopy (Procedure)
Regenerative Medicine
Histology Procedure
Esophageal Tissue
Glycine max
Keratinocyte
Squamous Transitional Epithelial Cell Count
Submucosal Route of Drug Administration
Buccal Surface

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