PMID: 9161886Mar 1, 1997Paper

Autologous lymphoblastoid cell lines stably transfected with Plasmodium falciparum circumsporozoite protein as targets in cytotoxic T-lymphocyte assays

Immunology Letters
A KumarStephen L Hoffman

Abstract

To produce cell lines that can be used as a continuous source of antigen presenting cells for stimulating T-cell lines and clones and as targets in cytotoxic T-lymphocyte (CTL) assays, we used a retroviral vector with a simian virus (SV40) early promotor to transfer a Plasmodium falciparum circumporozoite (PfCSP) gene into human EBV transformed B-lymphoblastoid cell lines (B-LCL). We herein report successful, stable transfection and cell surface expression of this gene, as confirmed by PCR, Western blot analysis and immunoelectron microscopy. One of three successfully transfected autologous cell lines expressed PfCSP on the cell surface and was lysed by CD8+ T-cell dependent CTL from a donor volunteer who had been immunized with irradiated P. falciparum sporozoites. Such cell lines should provide excellent tools for characterizing human CD8+ T-cell responses against Plasmodium sp. proteins.

References

Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A MalikS L Hoffman
Apr 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Y H KangR K Maheshwari
Apr 1, 1973·Virology·F L Graham, A J van der Eb

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Citations

May 26, 2005·The Journal of General Virology·Benno WölkDarius Moradpour

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