PMID: 3262710Oct 1, 1988Paper

Autologous tumor-specific cytotoxic T lymphocytes in the infiltrate of human metastatic melanomas. Activation by interleukin 2 and autologous tumor cells, and involvement of the T cell receptor

The Journal of Experimental Medicine
K ItohC M Balch

Abstract

TIL from metastatic melanoma proliferated by greater than 1,000-fold (840-3,675, mean 1,543) after 6 wk in culture of mixtures of TIL and tumor cells with rIL-2 alone. Cytolysis was restricted to autologous tumor cells. CD8+ T cells were the predominant population of TIL before and after expansion, and were primarily responsible for autologous tumor-specific CTL activity. No other rIL-2-activated lymphocytes from peripheral blood, lymph nodes with melanoma metastasis, or TIL from sarcoma or renal cell carcinoma had autologous tumor-specific CTL activity. There were few or no CD16+ NK cells in TIL from metastatic melanoma before or after incubation with rIL-2, respectively. However, TIL from sarcoma or renal cell carcinoma contained a substantial proportion of CD3-CD16+ NK cells, which increased in number in culture with rIL-2. Purified CD16+ NK cells as well as CD3+CD16- T cells from rIL-2-activated TIL of renal cell carcinoma displayed MHC-nonrestricted cytotoxicity. At the clonal level as determined by limiting dilution, 8 of 10 clones from melanoma TIL displayed cytotoxicity restricted to autologous tumor cells, while all 13 clones from renal cancer TIL equally lysed autologous and allogeneic tumor cells. Anti-T cell recepto...Continue Reading

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Citations

Mar 17, 1997·International Journal of Cancer. Journal International Du Cancer·T HoshinoK Itoh
Oct 10, 1998·International Journal of Cancer. Journal International Du Cancer·K KonoY Matsumoto
Feb 14, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y WadaM Kojiro
Jun 1, 1991·Cancer Metastasis Reviews·C D Platsoucas
Jul 1, 1993·Cancer Immunology, Immunotherapy : CII·A M NouriR T Oliver
Jul 1, 1993·Cancer Immunology, Immunotherapy : CII·A Uchida
Nov 1, 1993·Cancer Immunology, Immunotherapy : CII·D S HoonD L Morton
Jan 1, 1992·Cancer Immunology, Immunotherapy : CII·E B Reilly, G Antognetti
Jan 1, 1992·Cancer Immunology, Immunotherapy : CII·M A SalmeronK Itoh
Jan 1, 1992·Cancer Immunology, Immunotherapy : CII·M Apiranthitou-DrogariP Allavena
Jan 1, 2010·Cancer Immunology, Immunotherapy : CII·Adam DangoorRobert Hawkins
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