Autologous versus pooled human serum for articular chondrocyte growth

Journal of Orthopaedic Surgery
S MunirahB S Aminuddin

Abstract

To evaluate the effect of autologous human serum (AHS) versus pooled human serum (PHS) versus foetal bovine serum (FBS) for growth of articular chondrocytes and formation of chondrocytefibrin constructs. Experiments with monolayer culture expansion of human articular chondrocytes were performed using basic culture media supplemented with 10% AHS, PHS, or FBS. Growth kinetics and specific phenotypic expression of the serially expanded chondrocytes were evaluated. Large-scale culture expansion was used to obtain about 30 million cells to form chondrocyte-fibrin constructs. All constructs were implanted subcutaneously at the dorsum part of athymic nude mice for 8 weeks. The in vivo constructs were evaluated using histological and gene expression studies. The morphology of primary cultured chondrocytes (P0) was polygonal and became more elongated and larger after serial passages (P1, P2, and P3). This was comparable for AHS, PHS, and FBS. Total cell yields accumulated for AHS (28 million) and PHS (41 million) were significantly higher than those for FBS (4 million). After 8 weeks of implantation, in vivo chondrocyte-fibrin constructs demonstrated a glistening white and firm texture, comparable to normal hyaline cartilage. All const...Continue Reading

References

Oct 6, 1994·The New England Journal of Medicine·M BrittbergL Peterson
May 20, 2000·Clinical Orthopaedics and Related Research·L PetersonA Lindahl
Nov 9, 2000·Biomaterials·R G LeBaron, K A Athanasiou
Oct 18, 2001·Clinical Orthopaedics and Related Research·M BrittbergL Peterson
Apr 15, 2005·European Cells & Materials·S N RedmanC W Archer
Jul 5, 2005·Clinical Orthopaedics and Related Research·Jon E BrowneChristoph Erggelet
Sep 1, 2006·Cell and Tissue Research·Sriram V EleswarapuKyriacos A Athanasiou

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