PMID: 9442493Jan 1, 1997Paper

Autolysis and cell wall degradation in a choline-independent strain of Streptococcus pneumoniae

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
A SeverinA Tomasz

Abstract

Streptococcus pneumoniae has an auxotrophic requirement for choline, and choline residues that incorporate into the wall and membrane teichoic acids are intimately involved with the control of autolytic phenomena of this bacterium. We report here the re-examination of the role of choline in autolytic cell wall degradation using the choline-independent S. pneumoniae strain R6Cho- recovered from a heterologous cross with DNA from Streptococcus oralis. S pneumoniae Cho- cultured in choline-free medium grew with normal generation time but formed long chains, failed to undergo stationary-phase autolysis, and was also resistant to lysis induced by deoxycholate or penicillin. Cell walls produced under these conditions had reduced phosphorus content, contained no choline residues detectable by nuclear magnetic resonance, and had reduced binding capacity for the pneumococcal autolytic amidase, and complete hydrolysis of such walls by the amidase required prolonged incubation with high concentrations of the enzyme. Addition of choline to the growth medium reversed at these phenomena. High-performance liquid chromatography analysis of amidase digests of cell walls prepared from strain R6Cho- grown with or without choline produced identica...Continue Reading

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Citations

Feb 27, 1999·Current Opinion in Microbiology·E Tuomanen
Aug 1, 2008·The Journal of Biological Chemistry·Begoña MonterrosoMargarita Menéndez
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Jun 8, 2019·Microbiology Spectrum·Waldemar VollmerAlexander Tomasz

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