Autolysis of lactococci: detection of lytic enzymes by polyacrylamide gel electrophoresis and characterization in buffer systems.

Applied and Environmental Microbiology
Hilde M OstlieThor Langsrud

Abstract

Lactococcal strains were screened for bacteriolytic activity against Micrococcus luteus cells, lactococcal cells, and cell walls. Thirty strains were screened for bacteriolytic activity against cells and cell walls incorporated into agar medium. Enzymes from all strains hydrolyzed the substrates; however, the activity against Micrococcus cells was much higher than against Lactococcus cells or cell walls. Electrophoretic profiles of bacteriolytic activities of culture supernatants, sodium dodecyl sulfate-treated cell extracts, cell wall fractions, and cell extracts were analyzed in sodium dodecyl sulfate-polyacrylamide gels containing M. luteus cells or lactococcal cell walls as the substrate. The 22 strains tested contained two to five lytic bands in the culture supernatant, ranging in size between 32 and 53 kDa. The cell extracts, the sodium dodecyl sulfate-treated cell extracts, and the cell wall fractions revealed two lytic bands of 47 and 53 kDa. Effects of external factors on autolysis of some strains were determined in buffer systems. Optimal autolysis was observed in the exponential growth phase at pH 6.0 to 7.5 and at a temperature of 30(deg)C. Two of three strains tested seemed to contain a glycosidase, and all three s...Continue Reading

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Citations

Oct 1, 1996·Antonie van Leeuwenhoek·M J Gasson
Oct 22, 2005·Journal of Applied Microbiology·N RaddadiD Daffonchio
Dec 19, 2000·Journal of Applied Microbiology·R Cibik, M P Chapot-Chartier
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Jun 10, 2004·Journal of Applied Microbiology·N RaddadiD Daffonchio
Jul 1, 1997·Applied and Environmental Microbiology·G BuistJ Kok
Dec 24, 1997·Applied and Environmental Microbiology·J W SandersJ Kok
Mar 15, 2006·Applied and Environmental Microbiology·H R RiepeL L McKay

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