Automated 3D-Printed Microfluidic Array for Rapid Nanomaterial-Enhanced Detection of Multiple Proteins

Analytical Chemistry
Karteek KadimisettyJames F Rusling

Abstract

We report here the fabrication and validation of a novel 3D-printed, automated immunoarray to detect multiple proteins with ultralow detection limits. This low cost, miniature immunoarray employs electrochemiluminescent (ECL) detection measured with a CCD camera and employs touch-screen control of a micropump to facilitate automated use. The miniaturized array features prefilled reservoirs to deliver sample and reagents to a paper-thin pyrolytic graphite microwell detection chip to complete sandwich immunoassays. The detection chip achieves high sensitivity by using single-wall carbon nanotube-antibody conjugates in the microwells and employing massively labeled antibody-decorated RuBPY-silica nanoparticles to generate ECL. The total cost of an array is $0.65, and an eight-protein assay can be done in duplicate for $0.14 per protein with limits of detection (LOD) as low as 78-110 fg mL-1 in diluted serum. The electronic control system costs $210 in components. Utility of the automated immunoarray was demonstrated by detecting an eight-protein prostate cancer biomarker panel in human serum samples in 25 min. The system is well suited to future clinical and point-of-care diagnostic testing and could be used in resource-limited en...Continue Reading

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Citations

Jun 6, 2019·Annual Review of Biomedical Engineering·Arman NaderiAlbert Folch
Jun 7, 2019·Biofabrication·Amir K MiriAli Khademhosseini
Mar 25, 2019·Analytical Methods : Advancing Methods and Applications·Karteek KadimisettyJames F Rusling
Aug 19, 2020·Sensors·Mohamed SharafeldinJames F Rusling
Dec 18, 2019·Nanomedicine : Nanotechnology, Biology, and Medicine·F Raquel MaiaJoaquim M Oliveira
Jul 3, 2021·Biomedicines·James F Rusling, Robert J Forster
Aug 11, 2021·Sensors·Josephine Aidoo-BrownPedro Estrela
Nov 2, 2019·Analytical Chemistry·Iulia M LazarAlexandru C Lazar
Nov 5, 2019·Analytical Chemistry·Cheng MaJun-Jie Zhu
May 6, 2019·Analytical Chemistry·Mohamed SharafeldinJames F Rusling
Nov 18, 2018·ACS Sensors·Alexander LambertQuan Cheng

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