Automated brightfield break-apart in situ hybridization (ba-ISH) application: ALK and MALT1 genes as models

Methods : a Companion to Methods in Enzymology
Hiroaki NittaThomas M Grogan

Abstract

Cancer diagnosis can be a complex process, which takes consideration of histopathological, clinical, immunophenotypic, and genetic features. Since non-random chromosomal translocations are specifically involved in the development of various cancers, the detection of these gene aberrations becomes increasingly important. In recent years, break-apart (or split-signal) fluorescence in situ hybridization (FISH) has emerged as an advantageous technique to detect gene translocations on tissue sections. However, FISH assays are technically challenging and require specialized fluorescence microscopes. Furthermore, the FISH signal is not stable for long term archiving due to photo bleaching. Our objective was to demonstrate the feasibility of brightfield break-apart in situ hybridization (ba-ISH) for anaplastic lymphoma kinase (ALK) and mucosa-associated lymphoid tissue translocation protein 1 (MALT1) genes as models. ALK or MALT1 break-apart probes were labeled with digoxigenin (DIG) or 2,4-dinitrophenyl (DNP) on both sides of a known gene breakpoint region and the hybridization sites were visualized with the combination of alkaline phosphatase (AP)-based blue and red detection. Therefore, normal genes are detected as purple dots by mi...Continue Reading

References

Sep 29, 2005·Hematology·J J M van DongenA W Langerak
Apr 29, 2006·The Journal of Molecular Diagnostics : JMD·Roland A VenturaReiner Siebert
Dec 13, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Antonio C WolffUNKNOWN College of American Pathologists
Jun 6, 2008·Applied Immunohistochemistry & Molecular Morphology : AIMM·Jennifer C PatersonTeresa Marafioti
Feb 19, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Daniel PiriciRadu Stanescu
Oct 24, 2009·Advances in Anatomic Pathology·Munir R Tanas, John R Goldblum

❮ Previous
Next ❯

Citations

Jul 30, 2014·Cellular & Molecular Immunology·Patricia D A LimaB Anne Croy
Jun 13, 2014·The American Journal of Surgical Pathology·Jitsupa TreetipsatitYasodha Natkunam
Jun 8, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Hans-Ulrich SchildhausReinhard Büttner
May 19, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Hyojin KimJin-Haeng Chung
Jul 3, 2013·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Hiroaki NittaThomas M Grogan
Mar 20, 2015·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Toni-Maree RogersStephen B Fox
Sep 17, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Akihiko YoshidaHitoshi Tsuda
Jan 26, 2011·Histopathology·Philip Kluin, Ed Schuuring
Sep 30, 2014·Applied Immunohistochemistry & Molecular Morphology : AIMM·Ubaradka G SathyanarayanaLei Tang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.

Related Papers

Nature Reviews. Cancer
Chandan Kumar-SinhaArul M Chinnaiyan
Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
Bo HanRajal B Shah
Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
Dimitris IoannouDarren K Griffin
© 2021 Meta ULC. All rights reserved