Abstract
Cancer diagnosis can be a complex process, which takes consideration of histopathological, clinical, immunophenotypic, and genetic features. Since non-random chromosomal translocations are specifically involved in the development of various cancers, the detection of these gene aberrations becomes increasingly important. In recent years, break-apart (or split-signal) fluorescence in situ hybridization (FISH) has emerged as an advantageous technique to detect gene translocations on tissue sections. However, FISH assays are technically challenging and require specialized fluorescence microscopes. Furthermore, the FISH signal is not stable for long term archiving due to photo bleaching. Our objective was to demonstrate the feasibility of brightfield break-apart in situ hybridization (ba-ISH) for anaplastic lymphoma kinase (ALK) and mucosa-associated lymphoid tissue translocation protein 1 (MALT1) genes as models. ALK or MALT1 break-apart probes were labeled with digoxigenin (DIG) or 2,4-dinitrophenyl (DNP) on both sides of a known gene breakpoint region and the hybridization sites were visualized with the combination of alkaline phosphatase (AP)-based blue and red detection. Therefore, normal genes are detected as purple dots by mi...Continue Reading
References
Sep 29, 2005·Hematology·J J M van DongenA W Langerak
Apr 29, 2006·The Journal of Molecular Diagnostics : JMD·Roland A VenturaReiner Siebert
Jul 11, 2006·The Journal of Pathology·M LaaksoJ Isola
Dec 13, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Antonio C WolffUNKNOWN College of American Pathologists
Jul 13, 2007·Nature·Manabu SodaHiroyuki Mano
Jun 6, 2008·Applied Immunohistochemistry & Molecular Morphology : AIMM·Jennifer C PatersonTeresa Marafioti
Oct 24, 2008·Diagnostic Pathology·Hiroaki NittaThomas M Grogan
Feb 19, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Daniel PiriciRadu Stanescu
Sep 24, 2009·Haematologica·Anke van RijkHan J van Krieken
Oct 24, 2009·Advances in Anatomic Pathology·Munir R Tanas, John R Goldblum
May 13, 2010·Histopathology·Tomás García-CaballeroJens Mollerup
Citations
Jul 30, 2014·Cellular & Molecular Immunology·Patricia D A LimaB Anne Croy
Aug 6, 2014·Human Pathology·Samantha L KendrickLisa M Rimsza
Jun 13, 2014·The American Journal of Surgical Pathology·Jitsupa TreetipsatitYasodha Natkunam
Jun 8, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Hans-Ulrich SchildhausReinhard Büttner
Dec 15, 2012·World Journal of Urology·Gerald KlinglmairJosef Oswald
May 19, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Hyojin KimJin-Haeng Chung
Jul 3, 2013·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Hiroaki NittaThomas M Grogan
Mar 20, 2015·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Toni-Maree RogersStephen B Fox
Sep 17, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Akihiko YoshidaHitoshi Tsuda
Jan 26, 2011·Histopathology·Philip Kluin, Ed Schuuring
Oct 2, 2014·Head & Neck·Sung Min KimMyung-Ju Ahn
Sep 3, 2013·The Journal of Molecular Diagnostics : JMD·Wenjun ZhangLei Tang
Jan 29, 2013·American Journal of Clinical Pathology·Carlo ValentinoLisa Rimsza
Jul 23, 2014·Diagnostic Pathology·Lisa M RimszaThomas M Grogan
Feb 13, 2018·Expert Review of Molecular Diagnostics·Chiara C VolpiAnnunziata Gloghini
Sep 30, 2014·Applied Immunohistochemistry & Molecular Morphology : AIMM·Ubaradka G SathyanarayanaLei Tang