Automated cold temperature cycling improves in vitro platelet properties and in vivo recovery in a mouse model compared to continuous cold storage

Transfusion
Andrey SkripchenkoStephen J Wagner

Abstract

Platelets (PLTs) stored at cold temperatures (CTs) for prolonged time have dramatically reduced bacterial growth but poor survival when infused. A previous study demonstrated that human PLTs stored with manual cycling between 4 °C (12 hr) and 37 °C (30 min) and infused into severe combined immunodeficient (SCID) mice had survivals similar to or greater than those stored at room temperature (RT). In this study, the in vitro and in vivo properties of PLTs stored in an automated incubator programmed to cycle between 5 °C (11 hr) and 37 °C (1 hr) were evaluated. A Trima apheresis unit (n = 12) was aliquoted (60 mL) in CLX bags. One sample was stored with continuous agitation (RT), a second sample was stored at 4-6 °C without agitation (CT), and a third sample was placed in an automated temperature cycler with 5 minutes of agitation during the warm-up period (thermocycling [TC]). PLTs were assayed for several relevant quality variables. On Day 7, PLTs were infused into SCID mice and in vivo recovery was assessed at predetermined time points after transfusion. The glucose consumption rate, morphology score, hypotonic shock recovery level, and aggregation levels were increased and mitochondrial reactive oxygen species accumulations we...Continue Reading

References

Mar 1, 1973·Transfusion·G A BeckerR H Aster
May 1, 1983·Transfusion·T L SimonS Murphy
Mar 1, 1984·Transfusion·G RockP Tittley
Sep 27, 2003·Thrombosis Research·Jong Weon Choi
Jun 29, 2004·Clinical Therapeutics·Toufigh Gordi, Harry Khamis
Sep 28, 2006·Transfusion·Sherrill J SlichterEdward L Snyder
Oct 31, 2013·Shock·Kristin M ReddochAndrew P Cap
Aug 28, 2014·The Journal of Trauma and Acute Care Surgery·Prajeeda M NairAnand K Ramasubramanian

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Citations

May 3, 2016·Platelets·Peter A Smethurst
Oct 18, 2016·Current Opinion in Hematology·Richard O Francis
Oct 21, 2016·Transfusion Medicine·S Thomas
Oct 4, 2018·Current Opinion in Hematology·Shailaja HegdeJose A Cancelas
Feb 24, 2021·Veterinary Clinical Pathology·Ashleigh V LemonEmma H Hooijberg

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