Automated correlation and averaging of three-dimensional reconstructions obtained by electron tomography

Journal of Structural Biology
L G OfverstedtU Skoglund

Abstract

We have developed a least-squares refinement procedure that in an automated way performs three-dimensional alignment and averaging of objects from multiple reconstructions. The computer implementation aligns the three-dimensional structures by a two-step procedure that maximizes the density overlap for all objects. First, an initial average density is built by successive incorporation of individual objects, after a global search for their optimal three-dimensional orientations. Second, the initial average is subsequently refined by excluding individual objects one at a time, realigning them with the reduced average containing all other objects and including them into the average again. The refinement is repeated until no further change of the average occurs. The resulting average model is therefore minimally biased by the order in which the individual reconstructions are incorporated into the average. The performance of the procedure was tested using a synthetic data set of randomly oriented objects with Poisson-distributed noise added. The program managed well to align and average the objects at the signal/noise ratio 1.0. The increase in signal/noise ratio was in all investigated cases almost equal to the expected square root...Continue Reading

References

Jan 1, 1992·Ultramicroscopy·P PenczekJ Frank
Aug 1, 1988·Journal of Electron Microscopy Technique·M Radermacher
May 27, 1971·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R A Crowther
Jul 25, 1972·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·P F Gilbert
May 13, 1982·Nature·Y MauguenA Jack
Apr 1, 1995·Current Opinion in Structural Biology·J Frank
Jan 1, 1996·Journal of Structural Biology·S D FullerT S Baker

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Citations

Jul 19, 2005·The British Journal of Ophthalmology·D U BartschW R Freeman
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·Terrence G FreyMark H Ellisman
Jan 19, 2016·Cell Reports·Reetesh Raj AkhouriMats Wahlgren
Feb 28, 2006·Biology of the Cell·Cédric MessaoudiSergio Marco
Jan 4, 2005·Biochemistry. Biokhimii︠a︡·S MarcoJ-L Rigaud

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