Automated derivatization and analysis of malondialdehyde using column switching sample preparation HPLC with fluorescence detection

Journal of Separation Science
H LordMazen J Hamadeh

Abstract

Analyte derivatization is advantageous for the analysis of malondialdehyde (MDA) as a biomarker of oxidative stress in biological samples. Conventionally, however, derivatization is time consuming, error-prone and has limited options for automation. We have addressed these challenges for the solid phase analytical derivatization of MDA from small volume tissue homogenate samples. A manual derivatization method was first developed using Amberlite XAD-2 (12 mg) as the solid phase. Subsequently an automated column switching process was developed that provided simultaneous derivatization and extraction of the MDA-DH hydrazone product on a cartridge packed with XAD-2, followed by quantitative elution of the product to an analytical LC column (Waters NovoPak C18, 3.9 x 150 mm). The LOD was 0.02 microg/mL and recovery was quantitative. The method was linear (r(2) >0.999) with precision < 5% from the LOQ (0.06 microg/mL) to at least 35 microg/mL. The method was successfully applied to the analysis of small volume (30 microL) mouse tissue homogenate samples. Endogenous levels of MDA in the tissues ranged from 20 to 40 nmol/g tissue (ca. 0.1-0.2 microg/mL homogenate). Compared to conventional MDA analyses, the current method has advantag...Continue Reading

References

Apr 23, 2003·Analytical Chemistry·Thomas KeoughMartin P Lacey
Jul 26, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yang-Wei LinHuan-Tsung Chang
Nov 6, 2003·Medicine and Science in Sports and Exercise·M Christine RodriguezMark A Tarnopolsky
Jul 16, 2004·Journal of Neurotrauma·Joachim ScholppGabriele F E Noeldge-Schomburg
May 18, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Songmei GaoH T Karnes
Jun 11, 2005·European Journal of Mass Spectrometry·John M Halket, Vladimir G Zaikin
Jul 21, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gert EmmerechtsJef Rozenski
Sep 3, 2005·BMC Pulmonary Medicine·Matteo GoldoniAntonio Mutti
Sep 13, 2005·Pharmacology & Therapeutics·Michelle J HansenGary P Anderson
Oct 11, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tatsuya HigashiKazutake Shimada
Jan 4, 2006·Cellular and Molecular Life Sciences : CMLS·P Venditti, S Di Meo
Jan 24, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jun UeyamaKenzo Takagi
Jan 28, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jianjun MaoGuoquan Liu
Jan 31, 2006·Journal of the American Society for Mass Spectrometry·William J GriffithsJan Sjövall
Feb 2, 2006·Diabetes, Obesity & Metabolism·L E Fridlyand, L H Philipson
Apr 4, 2006·Muscle & Nerve·Douglas J MahoneyMark A Tarnopolsky
Jun 15, 2006·Journal of Neuro-oncology·Bartosz WoźniakGrzegorz Planutis
Jun 29, 2006·Annals of the New York Academy of Sciences·Li Li JiJose Vina
Jul 18, 2006·Analytical Chemistry·Chris A JakoberPeter G Green
Jul 29, 2006·Journal of Alzheimer's Disease : JAD·P I MoreiraC R Oliveira
Aug 2, 2006·Experimental Eye Research·Chung-Jung Chiu, Allen Taylor
Aug 4, 2006·Journal of Chromatographic Science·Elena E StashenkoJairo R Martínez
Aug 18, 2006·Journal of Alzheimer's Disease : JAD·Mark A Smith
Aug 30, 2006·European Journal of Anaesthesiology·R TuranB Dikmen
Sep 5, 2006·Journal of Pharmaceutical and Biomedical Analysis·D GrottoS C Garcia

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Citations

Jan 4, 2020·Journal of Analytical Toxicology·Kostya KartavenkaP Barry Ryan
Dec 17, 2020·Comprehensive Reviews in Food Science and Food Safety·Xuxia ZhouYongning Wu
Mar 7, 2013·Analytical Chemistry·Alicia J Kalafut-Pettibone, W Sean McGivern

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