Automated digital image quantification of histological staining for the analysis of the trilineage differentiation potential of mesenchymal stem cells

Stem Cell Research & Therapy
Benjamin EggerschwilerPaolo Cinelli

Abstract

Multipotent mesenchymal stem cells (MSCs) have the potential to repair and regenerate damaged tissues and are considered as attractive candidates for the development of cell-based regenerative therapies. Currently, there are more than 200 clinical trials involving the use of MSCs for a wide variety of indications. However, variations in their isolation, expansion, and particularly characterization have made the interpretation of study outcomes or the rigorous assessment of therapeutic efficacy difficult. An unbiased characterization of MSCs is of major importance and essential to guaranty that only the most suitable cells will be used. The development of standardized and reproducible assays to predict MSC potency is therefore mandatory. The currently used quantification methodologies for the determination of the trilineage potential of MSCs are usually based on absorbance measurements which are imprecise and prone to errors. We therefore aimed at developing a methodology first offering a standardized way to objectively quantify the trilineage potential of MSC preparations and second allowing to discriminate functional differences between clonally expanded cell populations. MSCs originating from several patients were differentia...Continue Reading

References

Apr 2, 1999·Science·M F PittengerD R Marshak
Aug 6, 2003·Blood·Pieternella S In 't AnkerHumphrey H H Kanhai
Nov 2, 2006·Genome Biology·Anne E CarpenterDavid M Sabatini
Dec 26, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Chris M van der Loos
Feb 7, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sun-Yong KimYun-Song Lee
Mar 21, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Rikke Riber-HansenTorben Steiniche
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Sep 4, 2012·International Journal of Molecular Medicine·P T NewtonC Farquharson
Aug 3, 2013·Medical Image Analysis·Anders EklundStephen M LaConte
Oct 23, 2013·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Peter StankoCestmir Altaner
Feb 19, 2016·IEEE Transactions on Medical Imaging·Shadi AlbarqouniNassir Navab
May 5, 2016·Cellular and Molecular Life Sciences : CMLS·Miaohua MoYaojiong Wu
Sep 14, 2016·IEEE Transactions on Medical Imaging·Youyi SongTianfu Wang
Sep 7, 2018·Nature·Yuki IkebuchiHiroshi Suzuki
Sep 23, 2018·Cell·Charles K F ChanMichael T Longaker

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Citations

Jan 23, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anna Xiao-Dan YuKarl Wah-Keung Tsim
May 31, 2020·Journal of Clinical Periodontology·Mohamed MekhemarKarim Fawzy El-Sayed
Sep 8, 2020·Stem Cell Research & Therapy·Young Hoon KimChang H Lee
Jul 22, 2021·Microscopy Research and Technique·Silvia GroissDagmar Brislinger

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Methods Mentioned

BETA
FCS
light microscopy

Clinical Trials Mentioned

NCT01218945

Software Mentioned

ImageJ
GraphPad
Cell Profiler
DIA
Gen 5 image prime
Fiji
Graph Pad Prism

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