Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles

Journal of Biotechnology
Hiroyuki OtaTadashi Matsunaga

Abstract

A novel, automated system, PNE-1080, equipped with eight automated pestle units and a spectrophotometer was developed for genomic DNA extraction from maize using aminosilane-modified bacterial magnetic particles (BMPs). The use of aminosilane-modified BMPs allowed highly accurate DNA recovery. The (A(260)-A(320)):(A(280)-A(320)) ratio of the extracted DNA was 1.9+/-0.1. The DNA quality was sufficiently pure for PCR analysis. The PNE-1080 offered rapid assay completion (30 min) with high accuracy. Furthermore, the results of real-time PCR confirmed that our proposed method permitted the accurate determination of genetically modified DNA composition and correlated well with results obtained by conventional cetyltrimethylammonium bromide (CTAB)-based methods.

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Citations

Jun 19, 2008·Journal of the Royal Society, Interface·Atsushi ArakakiTadashi Matsunaga
Dec 26, 2015·Colloids and Surfaces. B, Biointerfaces·Yoshiaki MaedaTsuyoshi Tanaka
Jan 24, 2012·Forensic Science International. Genetics·Chantal J Frégeau, Anick De Moors
Jun 21, 2019·Scientific Reports·Ana PeigneuxConcepción Jimenez-Lopez
May 17, 2017·Chemistry, an Asian Journal·Xuan-Hung PhamBong-Hyun Jun
May 14, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Tsuyoshi TanakaTadashi Matsunaga

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