PMID: 9441928Jan 27, 1998Paper

Automated electron tomography of large nuclear RNP (InRNP) particles--the naturally assembled complexes of precursor messenger RNA and splicing factors

Journal of Structural Biology
O MedaliaRuth Sperling

Abstract

Splicing of nuclear pre-mRNA is an important step in the regulation of gene expression as only correctly spliced mRNAs will be exported to the cytoplasm to function in protein synthesis. Nuclear RNA transcripts of split genes and their splicing products, as well as the general population of nuclear polyadenylated RNA, are packaged in multicomponent large nuclear ribonucleoprotein (lnRNP) particles. These lnRNP particles, which sediment at the 200S region in sucrose gradients, contain all U snRNPs required for pre-mRNA splicing and several protein splicing factors, including U2AF and the SR proteins and can thus be viewed as naturally assembled complexes of pre-mRNA and splicing factors. We have previously reconstructed the three-dimensional image of negatively stained individual lnRNP particles by automated electron tomography. The reconstruction revealed a compact structure, 50 nm in diameter, composed of four major subunits. Here we further analyzed the reconstructed models and the apparent connectivity between the subunits using a new rendering technique. The uniformity of the lnRNP particles was substantiated by measurement of the volume engulfed by their surface. This study further supports the model proposed for the packa...Continue Reading

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Citations

Jun 1, 2006·Pflügers Archiv : European journal of physiology·Victor Shahin
Oct 20, 2012·Amino Acids·Michael S ParkerSteven L Parker
Feb 28, 2003·Methods : a Companion to Methods in Enzymology·Francisco IborraDean A Jackson
Aug 6, 2002·Journal of Structural Biology·Ohad MedaliaRuth Sperling
Dec 20, 2002·Journal of Structural Biology·Oleg RaitskinRuth Sperling
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Binghui LiJoseph Sperling
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·C SchäferH Oberleithner
Sep 22, 2006·Molecular Biology of the Cell·David GrünwaldUlrich Kubitscheck
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Jun 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·O RaitskinR Sperling
Mar 16, 2007·Journal of Molecular Biology·Sara Cohen-KrauszJoseph Sperling
Nov 20, 2014·Computational and Structural Biotechnology Journal·Kinneret SheferRuth Sperling
Jan 3, 2006·Journal of Molecular Biology·Maia AzubelJoseph Sperling
Dec 14, 1999·The Journal of Biological Chemistry·M R LadomeryN D Hastie
Jan 15, 2019·Microscopy Research and Technique·Qingfeng MaYasuhito Ishigaki

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