PMID: 15385839Sep 24, 2004Paper

Automated, fast, and sensitive quantification of drugs in human plasma by LC/LC-MS: quantification of 6 protease inhibitors and 3 nonnucleoside transcriptase inhibitors

Therapeutic Drug Monitoring
Wolfgang Egge-JacobsenU Christians

Abstract

An analytic assay based on automated sample preparation and liquid chromatography (LC) coupled with electrospray mass spectrometry (ESI-MS) was developed for the quantification of 6 protease inhibitors (PIs) and 3 nonnucleoside reverse transcriptase inhibitors (NNRTIs). The 6 PIs, amprenavir, indinavir, ritonavir, lopinavir, nelfinavir, and saquinavir, as well as the three NNRTIs, nevirapine, efavirenz, and delavirdine, require a succinct analysis technique for therapeutic drug monitoring in HIV/AIDS patients. After protein precipitation, samples were loaded on a C8, 10 x 4-mm extraction column, washed, and, after activation of the column-switching valve, backflushed onto the 30 x 2.1 mm C8 analytic column. [M+H] ions were detected in the selected ion mode. A nonlinear fit (y(-1) = a + b/x, all r2 > 0.999) for amprenavir, indinavir, ritonavir, lopinavir, nelfinavir, and saquinavir and a linear fit (y = ax + b, all r2 > 0.999) for nevirapine, efavirenz, and delavirdine led to best regression. Absolute recoveries were as follows: PIs > 81%; NNRTIs > 76%. Interday and intraday precision were <12.5% for the PIs and <11.7% for the NNRTIs. Interday and intraday accuracy were <12.2% for the PIs and <14.9% for the NNRTIs. Limits of qua...Continue Reading

References

Oct 1, 1991·European Journal of Drug Metabolism and Pharmacokinetics·V P ShahR D McDowall
Feb 7, 1998·The Journal of Clinical Investigation·R B KimG R Wilkinson
Jan 23, 1999·Pharmacotherapy·K Z Rana, M N Dudley
Mar 19, 1999·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·L I Malaty, J J Kuper
Jan 27, 2000·JAMA : the Journal of the American Medical Association·C C CarpenterP A Volberding
Jun 6, 2000·Advanced Drug Delivery Reviews·G C Williams, P J Sinko
Jul 13, 2000·Reviews in Medical Virology·M Harris, J S Montaner
Nov 25, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·U ChristiansG I Kirchner
May 22, 2002·Therapeutic Drug Monitoring·Rolf P G Van Heeswijk
Jun 22, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C MarzoliniL A Decosterd
Aug 31, 2002·AIDS Research and Human Retroviruses·Edward P AcostaUNKNOWN Adult Pharmacology Committee of the AIDS Clinical Trials Group
Dec 31, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Michele L TurnerEdward P Acosta
Mar 26, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Valerie A FrerichsGene D Morse
Apr 23, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Katharina M Rentsch
Dec 25, 2004·Drug Metabolism and Pharmacokinetics·Lynda FrassettoLeslie C Floren

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Citations

Apr 13, 2007·Analytical and Bioanalytical Chemistry·Frank T Peters
May 24, 2007·Analytical and Bioanalytical Chemistry·Franck Saint-MarcouxPierre Marquet
Aug 29, 2012·The Journal of Infectious Diseases·Monica GandhiUNKNOWN Women's Interagency HIV Study
Jan 24, 2009·AIDS·Monica GandhiUNKNOWN Women's Interagency HIV Study (WIHS)
May 2, 2009·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Monica GandhiUNKNOWN Women's Interagency HIV Study
Jul 15, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jiangeng HuangYazen Alnouti
May 6, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·R ter HeineA D R Huitema
Oct 30, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Antonio D'AvolioGiovanni Di Perri
Sep 30, 2005·Rapid Communications in Mass Spectrometry : RCM·Therese KoalVolkhard Kaever
Jul 20, 2007·Rapid Communications in Mass Spectrometry : RCM·Anne-Kathrin GehrigJürgen Burhenne
Mar 1, 2006·Journal of Pharmaceutical and Biomedical Analysis·R YekkalaE Adams
Mar 8, 2005·Journal of Mass Spectrometry : JMS
Jul 27, 2005·Therapeutic Drug Monitoring·Patrick LemmerRobert Wennig

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