Automated fluorescent in situ hybridization (FISH) analysis of t(9;22)(q34;q11) in interphase nuclei

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Béla KajtárLászló Pajor

Abstract

For chronic myeloid leukemia, the FISH detection of t(9;22)(q34;q11) in interphase nuclei of peripheral leukocytes is an alternative method to bone marrow karyotyping for monitoring treatment. With automation, several drawbacks of manual analysis may be circumvented. In this article, the capabilities of a commercially available automated image acquisition and analysis system were determined by detecting t(9;22)(q34;q11) in interphase nuclei of peripheral leukocytes. Three peripheral blood samples of normal adults, 21 samples of CML patients, and one sample of a t(9;22)(q34;q11) positive cell-line were used. Single nuclei with correctly detected signals amounted to 99.6% of nuclei analyzed after exclusion of overlapping nuclei and nuclei with incorrect signal detection. A cut-off value of 0.84 mum was defined to discriminate between translocation positive and negative nuclei based on the shortest distance between signals. Using this value, the false positive rate of the automated analysis for negative samples was 7.0%, whereas that of the manual analysis was 5.8%. Automated and manual results showed strong correlation (R(2) = 0.985), the mean difference of results was only 3.7%. A reliable and objective automated analysis of lar...Continue Reading

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Citations

Oct 23, 2012·Journal of Biomedical Optics·Yuchen QiuHong Liu
Jun 24, 2014·TheScientificWorldJournal·Yessi JusmanNoor Azuan Abu Osman
Apr 9, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Donát AlpárBéla Kajtár
Jun 15, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Gábor PajorDonát Alpár
Nov 19, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Qinghua DuYi Ying
Jun 15, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Zenonas TheodosiouKleoniki Lyroudia
Nov 1, 2007·Journal of Microscopy·Shishir Shah
Mar 21, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·János SzöllosiAttila Tárnok
May 20, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Gábor SmukGábor Pajor

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