PMID: 8608197Oct 1, 1995Paper

Automated fluorimetric assay procedure for glucohydrolases using a routine centrifugal analyser assay of enzymes of lysosomal origin in plasma, II

European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
G GoiG Tettamanti

Abstract

The manual fluorimetric procedure, considered as a reference method for the determination of N-acetyl-beta-D-glucosaminidase, beta-D-glucuronidase and beta-D-galactosidase in human plasma, was automated as a routine method, using the IL Monarch centrifugal analyser. Using a liquid standard with a known enzyme content, the automated assay correlated fairly well with the reference manual method (r values very close to 1). Its analytical imprecision was much lower than that of the manual method. The automated assay of N-acetyl-beta-D-glucosaminidase, beta-D-glucuronidase and beta-D-galactosidase gave coefficients of variation of 5.7-6.9, 3.6-5.0 and 3.8-4.2%, respectively, detection limits of 4, 2 and 1 mU/l plasma respectively, and linear responses of up to 73, 8.4 and 0.9 U/l of plasma respectively. Furthermore, the method required only small volumes of undiluted plasma (4-10 microliters). This method appears to be reliable, sensitive, simple enough for routine analyses and as cost effective as the most common routine serum enzyme assays.

References

Dec 1, 1990·Biochemical Medicine and Metabolic Biology·G SeveriniM Tarquini
May 15, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·C T YuenR G Price
Jan 1, 1965·Advances in Clinical Chemistry·E J Van Kampen, W G Zijlstra
Dec 1, 1965·Clinica Chimica Acta; International Journal of Clinical Chemistry·J W Woollen, P Turner
Nov 30, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·A LombardoG Tettamanti
Jan 1, 1983·Advances in Enzyme Regulation·H GrunickeB Puschendorf
Apr 25, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·A RegleroJ A Cabezas
Dec 22, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·A LombardoG Tettamanti
Jun 15, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·A ViganòM Salmona
Jul 1, 1981·Acta diabetologica latina·E KohlerT A Good

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinica Chimica Acta; International Journal of Clinical Chemistry
A LombardoG Tettamanti
European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
G GoiG Tettamanti
Clinica Chimica Acta; International Journal of Clinical Chemistry
C BairatiA Lombardo
Clinica Chimica Acta; International Journal of Clinical Chemistry
A LombardoG Tettamanti
Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
Volkmar GieselmannKlaus Harzer
© 2021 Meta ULC. All rights reserved