Automated four-color DNA sequencing using primers assembled by hexamer ligation

Gene
T Kaczorowski, W Szybalski

Abstract

A procedure based on the assembly of sequencing primers by hexamer ligation and then using them in automated DNA sequencing is described. This method is based on a four-color fluorescent terminator chemistry. Sequencing ladders were analyzed using an ABI 373 DNA sequencer (Applied Biosystems, Foster City, CA, USA). The best results were obtained for primers assembled by ligation of four to ten hexamers. The accuracy of the method was estimated to be 99.5% up to 400 nt of the read sequence, and somewhat lower at 400-600 nt.

References

Dec 11, 1992·Science·J KieleczawaF W Studier
Apr 27, 1989·Nature·W R Brown
Jul 28, 1995·Science·R D FleischmannJ M Merrick
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L E KotlerL E Ulanovsky
Apr 1, 1996·Molecular Biotechnology·T Kaczorowski, M Sektas
Nov 7, 1996·Gene·T Kaczorowski, W Szybalski

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